| 高效液相色谱法测定复方樟脑乳膏中葡萄糖酸氯己定的含量 |
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| 引用本文： | 林蕊云. 高效液相色谱法测定复方樟脑乳膏中葡萄糖酸氯己定的含量[J]. 海峡药学, 2013, 0(12): 92-94 |
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| 作者姓名： | 林蕊云 |
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| 作者单位： | 福建省福清市药品检验所,福清350100 |
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| 摘 要： | 目的 建立复方樟脑乳膏中葡萄糖酸氯己定的含量测定方法.方法 采用高效液相色谱法,色谱柱为Lichrospher C18柱（4.6×250mm,5μm）,流动相为乙腈-甲醇-水-三乙胺（5：48：47：0.4）（用磷酸调节pH值至3.0）,检测波长为258nm,流速为1.0mL·min-1,柱温为30℃.结果 本法精密度高,稳定性好,葡萄糖酸氯己定浓度在5.82～58.16μg·mL-1范围内具有良好的线性关系（r=0.9999）;平均加样回收率99.0%,RSD为0.97%.结论 本方法简便,准确,重现性好,可作为该制剂的含量测定方法.
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| 关 键 词： | 复方樟脑乳膏 葡萄糖酸氯己定 高效液相色谱法 |
Determination of Chlorhexidine Gluconate in Compound Camphor Cream by HPLC |
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| LIN Rui-yun. Determination of Chlorhexidine Gluconate in Compound Camphor Cream by HPLC[J]. Strait Pharmaceutical Journal, 2013, 0(12): 92-94 |
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| Authors: | LIN Rui-yun |
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| Affiliation: | LIN Rui-yun(Fujian Province Fuqing Institute for Drug ControI,Fuqing 350300,China) |
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| Abstract: | OBJECTIVE To establish a method for determining of Chlorhexidine Gluconate in Compound Camphor Cream. METHODS HPLC method was earried on Liehraspher-C18（4. 6 × 250mm, 5μm） column was used with Acetonitrile-methanol-water-triethylamine （5：48 ： 47 ： 0. 4 ） （ abjusted pH to 3.0 by phosphoric acid） as mobile phase. The detection wavelength was set at 258nm. The flow rate was 1.0mL · min-1 and the column temperature at 30℃. RESULTS It was showed that the Chlorhexidine Gluconate had a linearty within the range of 5.82- 8. 16μg·mL-1 ,overage recovery was 99.0% ,RSD =0. 97%. CONCLUSIONS This method is simple,accurate and reliable, It can be used for Determination of Compound Camphor tincture. |
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| Keywords: | Compound Camphor Cream Chlorhexidine Gluconate HPLC |
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