| HPLC法测定肝甦颗粒中绿原酸含量 |
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| 引用本文： | 黄德福,黄仁杰,魏文增. HPLC法测定肝甦颗粒中绿原酸含量[J]. 海峡药学, 2013, 0(12): 109-111 |
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| 作者姓名： | 黄德福 黄仁杰 魏文增 |
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| 作者单位： | [1]福建省福州市传染病医院,福州350025 [2]福建卫生职业技术学院,福州350101 [3]福建医科大学药学院,福州350100 |
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| 基金项目： | 福州市科技重点项目（编号：2012-S-157-5） |
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| 摘 要： | 目的 建立测定肝颗粒中绿原酸含量的方法.方法 采用高效液相色谱法.色谱柱为WondaSil C18（4.6mm×150mm,5μm）,流动相为乙腈-0.1%磷酸水溶液（20：80,V/V）,流速为1.0mL·min-1,检测波长为327nm,柱温为25℃,进样量为20μL.结果 绿原酸的质量浓度线性范围为8.0～126.8μg·mL-1（r=0.9997,n=6）;平均加样回收率为98.6%,RSD为0.66%（n=9）.结论 本方法快速、简便、准确、重现性良好,可用于该制剂的质量控制.
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| 关 键 词： | HPLC 肝甦颗粒 绿原酸 |
Content Determination of Chlorogenic Acid in Gansu Granules by HPLC |
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| HUANG De-fu,HUANG Ren-jie,WEI Wen-zeng. Content Determination of Chlorogenic Acid in Gansu Granules by HPLC[J]. Strait Pharmaceutical Journal, 2013, 0(12): 109-111 |
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| Authors: | HUANG De-fu HUANG Ren-jie WEI Wen-zeng |
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| Affiliation: | 1. Fuzhou Infectious Disease Hospital, Fuzhou 350025, China ;2. Fujian Health College, Fuzhou 350101 ,China;3. Fujian Medical University, Fuzhou 350100,China) |
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| Abstract: | OBJECTIVE To establish the method for the content determination of Chlorogenic acid in Gansu granules. METHODS HPLC method was adopted. Chromatographic conditions were as follows： WondaSil C18（4. 6mm × 150mm,5μm） column was used with mobile phase consisted of acetonitrile-0. 1% phosphoric acid（20： 80 ,V/V）at a flow rate of 1.0mL·min-1 under a detection wavelength at 327nm. The column temperature was 25℃, the injection volume was 20μL. RESULTS The concentration of chlorogenic acid was linear in the range of 8 - 126.8μg. mL-1 （r =0.9997,n =6） with an average recovery rate of 98.6%,RSD =0.66% （n =9） CONCLUSION A rapid, simple, accurate and reproducible method for the quality control of the preparation is established. |
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| Keywords: | HPLC Gansu granule Chlorogenic acid |
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