睾酮对血管内皮细胞表达血栓调节蛋白的影响

目的　观察睾酮对内皮细胞表达血栓调节蛋白的作用。方法　用不同浓度的睾酮 (2、6、10、10 0、30 0、4 0 0μg L)处理传代培养内皮细胞株ECV 30 4细胞 2 4h后 ,用四唑盐比色试验检测细胞活力 ;用酶联免疫吸附法检测上层培养液中的可溶性血栓调节蛋白及细胞总血栓调节蛋白的含量。结果　 2、6、10 μg L的睾酮均促进细胞表达血栓调节蛋白 (2 .89± 0 .96vs1.77± 0 .13,3.0 8± 0 .77vs1.77± 0 .13,2 .72± 0 .4 5vs1.77± 0 .13) ,其中以 6 μg L的睾酮作用最大 ,高浓度的睾酮对血栓调节蛋白的表达没有作用 ,不同浓度的睾酮对可溶性血栓调节蛋白的分泌均没有作用。结论　睾酮促进细胞血栓调节蛋白表达增加 ,可增强细胞的抗凝能力。

Effect of testosterone on the synthesis of thrombomodulin in cultured vascular endothelial cells

Objective To observe the effect of testosterone on the synthesis and secretion of thromobomodulin in cultured endothelial cells.Methods Endothelial cells were incubated with different concentrations of testosterone(2、6、10、100、300、400 μg/L) for 24 hours.MTT test was carried out to evaluate the cell growing state and ELISA test was conducted to analyze the cell expression of thrombomodulin and the concentration of soluble thrombomodulin(sTM) in the culture medium.Results Testosterone had no effect on the secretion of sTM, but promoted the expression of TM in cells at 2 μg/L,6 μg/L and 10 μg/L(2.89±0.96 vs 1.77±0.13, 3.08±0.77 vs 1.77±0.13, 2.72±0.45 vs 1.77±0.13).Conclusion Testosterone's effect on the expression of TM in cells suggests that testosterone has the ability of anticoagulation, and may in part contribute to the difference in thromobomodulin concentration between different genders. The low levels of testosterone may be a contributor of the high incidence of cardiovascular events in meddle-aged males.