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First identification of Neospora caninum by PCR in aborted bovine foetuses in Romania
Authors:Ovidiu Şuteu  Adriana Titilincu  David Modrý  Andrei Mihalca  Viorica Mircean  Vasile Cozma
Affiliation:1. Department of Parasitology and Parasitic Diseases, University of Agricultural Science and Veterinary Medicine, Faculty of Veterinary Medicine, 3-5 M?n??tur Street, Cluj Napoca, Romania
2. Institute of Parasitology, Czech Academy of Sciences, ?eské Budějovice, Czech Republic
Abstract:The first identification of Neospora caninum infection in aborted bovine foetuses in Romania is reported. Nine aborted foetuses were collected from a dairy farm. The foetal age of foetuses was between 3 and 7 months. A 7-month-old foetus was mummified. N. caninum DNA Nc-5 region was amplified from samples extracted from brain tissues of three (33.3%) aborted foetuses. The foetal ages of PCR positive foetuses were 3, 4 and 7 months (mummified). No specific lesions or tissue cysts were found to the histological examination, because of advanced autolysis of brains.
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