跌打活血散气相色谱指纹图谱

目的:建立跌打活血散气相色谱指纹图谱,为制剂的质量评价提供参考。方法:建立制剂GC指纹图谱,使用中药色谱指纹图谱相似度评价系统(2008版)计算相似度;采用GC-MS法,通过对照品比对,NIST谱库及文献检索对共有峰进行指认,并与对照药材比对确定其来源。结果:方法学考察显示建立的GC指纹图谱精密度、重复性、稳定性均可满足指纹图谱分析的需要;指纹图谱中呈现20个共有峰,对其中的17个进行了指认;14批样品的指纹图谱相似度评价结果在0.564~0.986,表明不同样品的质量差异较大。结论:建立的GC指纹图谱方法快速、简便,能够全面反映跌打活血散中化学成分信息,可用于跌打活血散质量评价。

GC Fingerprint Analysis of Dieda Huoxue Powder

Objective: To establish a GC chromatographic fingerprint analysis method for the quality evaluation of Dieda Huoxue powder. Method: The fingerprint chromatography was performed by GC. The similarity was evaluated with Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (version 2008). Common peaks were identified by GC-MS through the comparison with the references, literature, and/or NIST database. Meanwhile, their origins were determined through the comparison with reference drugs. Result: The precision, repeatability and stability of the established method can meet the requirements of fingerprint chromatography analysis. There were 20 common peaks in the GC fingerprint, among which 17 peaks were identified. The similarity of 14 batches of samples ranged from 0.564 to 0.986, indicating greater differences in the qualities among the samples. Conclusion: The developed method could provide the comprehensive information about the chemical components in Dieda Huoxue powder in a rapid and convenient manner. It could be used in quality evaluation of the drug.