| 七氟烷抑制人乳腺癌细胞系MCF-7增殖、迁移和侵袭 |
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| 引用本文： | 王盛华,付民,赵高峰. 七氟烷抑制人乳腺癌细胞系MCF-7增殖、迁移和侵袭[J]. 基础医学与临床, 2019, 39(11): 1597-1602 |
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| 作者姓名： | 王盛华 付民 赵高峰 |
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| 作者单位： | 南阳市第二人民医院 麻醉科,河南 南阳,473000;南阳市第二人民医院 肿瘤科,河南 南阳,473000;郑州大学第一附属医院 普外三科,河南 郑州,450000 |
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| 摘 要： | 目的研究七氟烷对人乳腺癌细胞(MCF-7)增殖、迁移和侵袭的影响及其机制。方法用Western blot检测MCF-7细胞中高迁徙率族蛋白1 HMGB1的表达;miR-34a组(转染miR-34a mimics)、miR-con组(转染miR-con)、七氟烷处理+anti-miR-con组(转染anti-miR-con)及七氟烷处理+anti-miR-34a组(转染anti-miR-34a),均用脂质体法转染至MCF-7细胞;RT-qPCR检测细胞miR-34a表达;MTT法检测细胞增殖;Transwell小室法检测细胞迁移和侵袭;双荧光素酶报告基因实验检测细胞荧光活性。结果与对照组相比,1.7%的七氟烷处理的细胞中miR-34a表达显著升高(P<0.05),HMGB1表达显著降低(P<0.05),且细胞增殖、迁移和侵袭均显著下调(P<0.05);过表达miR-34a可抑制MCF-7细胞增殖、迁移和侵袭,敲减miR-34a可降低七氟烷对MCF-7细胞增殖、迁移和侵袭的抑制作用。miR-34a可明显降低野生型HMGB1的细胞荧光活性,且负向调控HMGB1的表达。结论七氟烷可抑制人乳腺癌细胞增殖、迁移和侵袭。
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| 关 键 词： | 七氟烷 乳腺癌 miR-34a/HMGB1通路 |
Sevoflurane inhibits proliferation,migration and invasion of human breast cancer cell line MCF-7 |
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| WANG Sheng-hua,FU Min,ZHAO Gao-feng. Sevoflurane inhibits proliferation,migration and invasion of human breast cancer cell line MCF-7[J]. Basic Medical Sciences and Clinics, 2019, 39(11): 1597-1602 |
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| Authors: | WANG Sheng-hua FU Min ZHAO Gao-feng |
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| Affiliation: | (Department of Anesthesiology, Nanyang Second People s Hospital, Nanyang 473000;Department of Oncology, Nanyang Second People s Hospital, Nanyang 473000;Department of general surgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, China) |
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| Abstract: | WANG Sheng-hua;FU Min;ZHAO Gao-feng(Department of Anesthesiology, Nanyang Second People s Hospital, Nanyang 473000;Department of Oncology, Nanyang Second People s Hospital, Nanyang 473000;Department of general surgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, China) |
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| Keywords: | sevoflurane breast cancer miR-34a/HMGB1 pathway |
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