| Institution: | 1. Graduate School of Fisheries Sciences, Hokkaido University, 3-1-1 Minato-Cho, Hakodate, Hokkaido, 041-8611, Japan;2. Zhejiang Province Joint Key Laboratory of Aquatic Products Processing, Institute of Seafood, Zhejiang Gongshang University, Hangzhou, China;3. Marukyo Bio Foods, 4-18-18 Chuo, Wakkanai, Hokkaido, 097-0022, Japan;4. Sapporo Fine Chemical Research Center, Marukyo Bio Foods, Nishimiyanosawa 4-2-1-40, Teine, Sapporo, Hokkaido, 006-0004, Japan;5. Faculty of Fisheries Sciences, Hokkaido University, 3-1-1 Minato-Cho, Hakodate, Hokkaido, 041-8611, Japan |
| Abstract: | Owing to the increasing popularity of chondroitin sulfate (CS) for joint pain treatment, the CS-production industry has been producing an increasing amount of waste, which includes type II collagen, non-collagenous proteins, and residual CS. To effectively utilize these resources, we intended to develop new products from the by-product of skate chondroitin sulfate production (BP-sCS). In this study, we examined the antioxidant and fibroblast-activating properties of BP-sCS, intending to apply it for a wound-healing promoter. BP-sCS exhibited ABTS and DPPH radical scavenging activities, protected L929 fibroblasts from H2O2- or AAPH-induced oxidative stress, and scavenged intracellular reactive oxygen species. Moreover, BP-sCS promoted L929 fibroblast proliferation/metabolism and stimulated collagen deposition into the extracellular matrix. In addition, BP-sCS counteracted AAPH-induced oxidative stress damage that inhibited fibroblast migration. These effect were attributed to the cooperation among the molecules of BP-sCS, namely, type II collagen peptides, non-collagenous peptides, and CS polysaccharides. Our findings indicate that BP-sCS has the potential as a novel wound-healing promoter. This study is the first step toward the realization of a sustainable CS-production industry by waste utilization in healthcare products. |
