| 美洲蟑螂重组BCG-Arg变应原致BALB/C小鼠的免疫原性评估 |
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| 引用本文： | 张玉振,梁建卫,周珍文. 美洲蟑螂重组BCG-Arg变应原致BALB/C小鼠的免疫原性评估[J]. 山东生物医学工程, 2011, 0(3): 188-190 |
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| 作者姓名： | 张玉振 梁建卫 周珍文 |
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| 作者单位： | [1]广州市干部疗养院,广州510530 [2]广州市妇女儿童医疗中心,广州510120 |
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| 基金项目： | 国家自然科学基金资助项目（60471003） |
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| 摘 要： | 对表达美洲蟑螂Arg变应原的BCG进行免疫原性评估,为BCG脱敏疫苗的研制奠定基础。106CFU重组BCG-Arg皮下注射免疫BALB/C小鼠,分别于免疫0、2、4、6、8周收集血清。使用纯化的重组Arg包被ELISA板,ELISA检测Arg特异性IgG及IgG1I、gG2a亚类。重组BCG-Arg皮下注射免疫BALB/C小鼠2周后,血清Arg特异性IgG显著高于对照组（P〈0.05）,实验组IgG1及IgG2a也显著高于对照组（P〈0.05）,IgG2a升高更明显。重组美洲大蠊BCG-Arg具免疫原性,并且诱导Th1优势免疫应答。
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| 关 键 词： | 美洲大蠊 精氨酸激酶 卡介苗 变应原 疫苗 免疫应答 |
Immunogenicity of Recombinant Periplaneta Americana BCG-Arg Allergen |
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| ZHANG Yuzhen,LIANG Jianwei,ZHOU Zhenwen. Immunogenicity of Recombinant Periplaneta Americana BCG-Arg Allergen[J]. Shandong Journal of Biomedical Engineering, 2011, 0(3): 188-190 |
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| Authors: | ZHANG Yuzhen LIANG Jianwei ZHOU Zhenwen |
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| Affiliation: | 1.Guangzhou Carde Sanatorium,Guangzhou 510530,China;2.Guangzhou Women and Children Medical Care,Guangzhou 510120) |
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| Abstract: | To evaluate the Immunogenicity of recombinant Periplaneta Americana BCG-Arg allergen,and to provide the basis for developing BCG allergen vaccine.BALB/C mice were immunized subcutaneously by 106CFU recombinant BCG-Arg,sera were collected at 0,2,4,6,8 weeks.Arg specific IgG,IgG1,IgG2a were measured by ELISA using purified recombinant Arg antigen.In BCG-Arg immunized group,Arg specific IgG rose quickly after 2 weeks,there was significant difference between the experimental and control groups（P0.05）,Arg specific IgG1,IgG2a were also higher than that of control groups（P0.05）,with IgG2a more significantly.Recombinant Periplaneta Americana BCG-Arg allergen has immunogenicity and it can induce Th1 dominant immune response. |
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| Keywords: | Periplaneta Americana Arginine kinase Bacille calmette-Guerin vaccine Allergen Vaccine Immune response |
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