壳聚糖水凝胶与星形胶质细胞体外生物相容性评价的实验研究

研究壳聚糖水凝胶材料与星形胶质细胞的体外生物相容性,初步探讨壳聚糖水凝胶作为神经组织工程支架材料的可行性。利用氯化壳聚糖、β-甘油磷酸钠和羟乙基纤维素制备壳聚糖水凝胶,MTT法评价其细胞毒性;体外培养鉴定新生Wistar大鼠脑皮层星形胶质细胞;壳聚糖水凝胶与星形胶质细胞体外共培养,观察星形胶质细胞在材料上的生长;MTT法检测接种后1、35、、7d的细胞增殖度。体外成功制备壳聚糖水凝胶,该材料无细胞毒性;体外分离、培养得到状态良好的星形胶质细胞;体外星形胶质细胞在材料上培养呈星形,生长良好,有分支状突起形成;MTT结果表明,材料-细胞共培养组中的细胞增殖度明显高于单纯细胞组（P〈0.001）。壳聚糖水凝胶与星形胶质细胞在体外具有良好的生物相容性,有望作为神经组织工程支架材料。

The Biocompatibility Evaluation of Chitosan Hydrogel and Astrocytes in Vitro

To study the biocompatibility of chitosan hydrogel with astr ocytes in vitro,and to explore its feasibility as scaffold material in neural t issue engineering.Chitosan chloride,β-glycerol phosphate,and hydrox yethyl cellulose were used as raw materials to prepare chitosan hydrogel,the cy t otoxicity of the material was evaluated by the degree cell proliferation;astroc ytes were isolated from the cerebral cortex of neonatal Wistar rats;astrocytes were co-cultured with chitosan hydrogel,the morphological changes of astrocytes was observed under light microscope;the cell vitality was detected by MTT assay on the day of 1,3,5 and 7.Results showed that the innoxious chitosan hydroge l were succes sfully prepared;the isolated astrocytes were star-like,in good condition with branch formation processes;the astrocytes on chitosan hydrogel group had bette r cell proliferation than the astrocytes group（P0.001）.The ch itosan hydrogel has good biocompatibility with astrocytes in vitro,it has a ch ance to be used as neural tissue engineering scaffold material in the near futur e.