从肿瘤转移相关因子探讨非小细胞肺癌气虚血瘀证的病理基础

目的 观察非小细胞肺癌气虚血瘀证患者外周血中肿瘤转移相关因子的表达情况。

Pathological Basis of Qi-deficiency and Blood Stasis Syndrome in Patients with Non-small Cell Lung Cancer Viewing from Tumor Metastasis Related Factors

Objective To observe the expressions of tumor metastasis related factors(TMRF)in peripheral blood of patients with non-small cell lung cancer(NSCLC)for exploring the molecular mechanism for genesis of qi-deficiency and blood stasis(QDBS)syndrome in patients.Methods Eighty selected NSCLC patients of stage ⅡB/Ⅲ were differentiated into the QDBS group and the non-QDBS group according to the Chinese medicine syndrome differentiation criteria,40 in each group.The serum levels of vascular endothelial growth factor(VEGF),endostatin(ES)and soluble intercellular adhesive molecule-1(sICAM-1)in patients were detected by ELISA,and the expression of adhesive molecule CD_44 in peripheral blood was determined using flow cytometry.Results Serum levels of VEGF(1002.78±312.08 ng/L),ES(120.88±20.00 μg/L),sICAM-1(531.78±213.37 μg/L)and CD_44(136.65±29.60)were significantly higher in patients of QDBS group than in patients of non-QDBS group(653.18±318.99 ng/L,98.29±23.92 μg/L,409.36±167.65 μg/L and 98.46±20.64,respectively,P<0.01).Conclusion Objective inner links are found between the QDBS syndrome and TMRF in NSCLC patients;serum levels of VEGF,ES,sICAM-1 and CD_44 can be served as the microcosmic basis for QDBS syndrome differentiation.