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Quantitative glycoproteomics of high-density lipoproteins |
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| Authors: | Xinyu Tang Maurice Wong Jennyfer Tena Chenghao Zhu Christopher Rhodes Qingwen Zhou Anita Vinjamuri Armin Oloumi Sucharita Boddu Guillaume Luxardi Emanual Maverakis Carlito B. Lebrilla Angela M. Zivkovic |
| Affiliation: | Department of Nutrition, University of California, Davis, One Shields Ave., Davis CA 9516 USA.; Department of Chemistry, University of California, Davis, One Shields Ave., Davis CA 95616 USA ; Department of Dermatology, University of California, Davis School of Medicine, 3301 C Street, Suite 1400, Sacramento CA 95816 USA |
| Abstract: | In this work, we developed a targeted glycoproteomic method to monitor the site-specific glycoprofiles and quantities of the most abundant HDL-associated proteins using Orbitrap LC-MS for (glyco)peptide target discovery and QqQ LC-MS for quantitative analysis. We conducted a pilot study using the workflow to determine whether HDL protein glycoprofiles are altered in healthy human participants in response to dietary glycan supplementation.The optimized HDL glycoproteomics method was sensitive enough to detect the effects of dietary supplements on HDL protein glycoprofiles even in a small sample size. |
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