黄芩水提取物对急性肺损伤大鼠的保护作用及其与胆碱能抗炎通路的相关性研究

目的探讨黄芩水提取物对急性肺损伤大鼠药效学指标及胆碱能抗炎通路的影响。方法黄芩水提取物ig给药1周后,大鼠尾iv给予脂多糖(LPS)建立急性肺损伤模型,观察各组大鼠基本情况,分别于注射LPS 1、2、4 h测定大鼠肛温,测定血清肿瘤坏死因子-α(TNF-α)、NO、乙酰胆碱(Ach)水平,同时取肺组织测定湿质量与干质量比值,制备肺组织匀浆,测定匀浆液中乙酰胆碱转移酶(ChAT)、乙酰胆碱酯酶(AChE)活性。结果与模型组比较,黄芩水提取物高、低剂量组及加兰他敏组均不同程度地缓解肺损伤大鼠炎症反应的发生,其中黄芩水提取物高剂量组在造模后2 h时能显著减轻大鼠肺组织水肿,减轻体温变化幅度,降低血清NO水平,提高血清Ach水平(P<0.05、0.01);同时降低血清TNF-α的量,增强肺组织匀浆液中ChAT的活性,但差异无统计学意义。与对照组比较,黄芩水提取物单独作用于正常大鼠时,上述各项指标均无明显差异。结论黄芩水提取物能提高急性肺损伤大鼠血清Ach水平,降低血清TNF-α、NO水平,抑制炎症反应进一步恶化,但对与Ach合成分解相关酶ChAT及AchE活性的影响并不显著,因此黄芩水提取物对胆碱能抗炎通路的影响可能是通过促进Ach释放发挥作用的,而与Ach的生物合成无关。

Correlation between protection of Scutellaria baicalensis extract on rats'acute lung injury and cholinergic anti-inflammatory pathway

Objective To explore the influence of Scutellaria baicalensis water extract(SBWE) on cholinergic anti-inflammatory pathway through acute lung injury(ALI) model.Methods The ALI model was set up by tail iv injection of lipopolysaccride(LPS) after ig administration SBWE for a week and then to observe the basis situations of rats in each group.The rectal temperature was measured after injected with LPS for 1,2,and 4 h,respectively.The changes of TNF-α,NO,and Ach levels in serum and ChAT and AChE activities in pulmonary tissues homogenate were detected by determining the lung wet/dry weight ratio.Results Compared with the model group,the high-and low-dose groups of SBWE as well as the group with galathamine showed the different degrees of protection on inflammatory response.Among them the high-dose group of SBWE could reduce the edema of lung tissues,the magnitude of body temperature changes,and the NO level in serum,while improve Ach content in serum significantly(P<0.05,0.01) 2 h after model set up.Besides,it could reduce TNF-α content in serum and increase the activity of ChAT in the lung tissue homogenate,but there was no statistical significance.Compared with the control group,no obvious difference of indexes mentioned above was observed between the control group and the group with SBWE merely.Conclusion SBWE could increase the Ach level in serum of ALI rats,decrease TNF-α content and NO levels,and effectively inhibit the further deterioration of inflammatory response.But there is no significant influence on Ach synthesis and decomposition related enzyme,ChAT and AChE activities.Thus effect of SBWE on cholinergic anti-inflammatory pathway may be due to indirectly promoting the release of Ach but not relating to the biosynthesis of Ach.