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| 亲溶酶体剂——十二烷基吗啡啉对白血病骨髓净化的实验研究 | |
| 引用本文： | 尹淑萍,丁立,杨纯正,褚建新,王军,李卫萍. 亲溶酶体剂——十二烷基吗啡啉对白血病骨髓净化的实验研究[J]. 中国医学科学院学报, 1990, 12(6): 405-409 |
| 作者姓名： | 尹淑萍 丁立 杨纯正 褚建新 王军 李卫萍 |
| 作者单位： | 血液学研究所 天津(尹淑萍，丁立，杨纯正，褚建新，王军)，血液学研究所 天津(李卫萍) |
| 基金项目： | 血液学研究所青年科学基金 |
| 摘 要： | 亲溶酶体剂——十二烷基吗啡啉(N-dodecyl morpholine,NDM)体外对小鼠白血病L_(7811)细胞系细胞有明显的杀灭作用,其100％杀死10~6个/mlL_(7811)细胞浓度为15mg/ml。用此浓度NDM处理含5％L_(7811)细胞的615小鼠骨髓细胞后回输给615小鼠,对照组存活天数为16.3±1.78d,溶剂组为15.8士2.32d,而NDM处理组可长期存活(>40d)。615小鼠在接受致死剂量(800rad)照射后输注经NDM处理的含5％L_(7811)细胞的615小鼠骨髓细胞,小鼠不死于白血病而死于放射病,当输注细胞数加倍时,小鼠存活期从8.75土1.13d延长到10.68±0.99d(P<0.01)。 |
| 关 键 词： | 亲溶酶休剂 十二烷基吗啡啉 白血病骨髓净化 |
Purging of Leukemic Cells from Mouse Marrow and Tumor Suspensions with Lysosomotropic Agent N-Dodecyl Morpholine |
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| Yin Shuping,et al. Purging of Leukemic Cells from Mouse Marrow and Tumor Suspensions with Lysosomotropic Agent N-Dodecyl Morpholine[J]. Acta Academiae Medicinae Sinicae, 1990, 12(6): 405-409 | |
| Authors: | Yin Shuping et al |
| Affiliation: | Institute of Hematology, Tianjin. |
| Abstract: | The lysosomotropic agent N-dodecyl morpholine (NDM) showed a marked killing of mice leukemic cells in vitro: 100% killing of L7811 leukemic cells (10(6) cells/ml) at a concentration of 15 micrograms/ml. Bone marrow of 615 mice containing 5% L7811 leukemic cells was treated with NDM and then infused into normal 615 mice. Mean survival times (MST) were 16.3 +/- 1.78 days and 15.8 +/- 2.32 days for control group and solvent group respectively, while the MST of groups treated with NDM were more than 40 days. When the marrow was infused into lethally irradiated mice, those mice receiving NDM-purged marrow died of radiation disease instead of leukemia. But when the number of marrow cells infused was increased to 2 x 10(6) cells/mouse, the mice survived for significantly longer times than did control group mice or those of the group infused with 10(6) cells/mouse. |
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