| 人胃癌裸鼠胃原位种植转移模型的建立和改进 |
| |
| 引用本文： | 孙永强,臧宏,王强,孙溪,肖忠标.人胃癌裸鼠胃原位种植转移模型的建立和改进[J].南通医学院学报,2005,25(3):169-170,173. |
| |
| 作者姓名： | 孙永强 臧宏 王强 孙溪 肖忠标 |
| |
| 作者单位： | 第二军医大学长征医院普外科,上海,200003 |
| |
| 摘 要： | 目的：建立人胃癌裸鼠胃原位种植转移模型。方法：用人胃癌细胞SGC-7901悬液接种于裸鼠皮下，形成稳定传代的皮下移植瘤。将皮下移植瘤组织再接种于裸鼠胃浆肌层，观察移植肿瘤的生长状况、移植成功率和自发转移的发生率及组织学变化等生物学特性。结果：肿瘤组织块保留了人胃腺癌的组织学特点，人胃癌裸鼠原位移植模型成瘤率为100％(20／20)，肝脏转移率为45％(9／20)。结论：人胃癌裸鼠原位移植瘤模型的生物学行学与人胃癌自然生长和转移过程相似，为研究人胃癌转移机制提供了理想的模型。
|
| 关 键 词： | 转移模型 原位种植 人胃癌细胞SGC-7901 裸鼠原位移植模型 原位移植瘤模型 皮下移植瘤 移植瘤组织 生物学特性 组织学变化 组织学特点 肿瘤组织块 胃浆肌层 生长状况 移植肿瘤 人胃腺癌 转移过程 生物学行 转移机制 再接种 |
| 文章编号： | 1000-2057(2005)03-0169-03 |
Establishment and improvement of orthotopic transplantation of human gastric tumor metastasis model in nude mice |
| |
| SUN Yongqiang,ZANG Hong,WANG Qiang,et al.Establishment and improvement of orthotopic transplantation of human gastric tumor metastasis model in nude mice[J].ACTA Academiae Medicinae Nantong,2005,25(3):169-170,173. |
| |
| Authors: | SUN Yongqiang ZANG Hong WANG Qiang |
| |
| Abstract: | Objective:To establish an orthotopic transplantation gastric tumor model in nude mice.Methods:Tumor cell line SGC-7901 of human gastric adenocarcinoma was inoculated subcutaneously into nude mice to develop implant tumor,and then intact tumor tissue was collected and implanted orthotopically into the nude mice stomach.Tumor growth,the rate of tumor survival and spontaneous metastasis was carried out,and the histological characteristics of transplantated tumors were also investigated.Results:The transplantation tumors resemble the original human gastric adenocarcinomoa histologically.The rate of tumor survival were 100%,and the liver metastases rate of orthotopic transplantation were 45% in the nude mice.Conclusion:In the growth and metastasis orthotopic transplantation model is similar to human gastric cancer. |
| |
| Keywords: | Human gastric tumor Tumor cells line SGC-7901 Nude mice Orthotopic transplantation Metastasis model |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
