Bcl-2及Bax表达与COPD大鼠骨骼肌细胞凋亡的相关性

目的探讨慢性阻塞性肺疾病(COPD)模型大鼠骨骼肌Bc l-2、Bax表达与骨骼肌细胞凋亡的关系。方法 100只健康W istar大鼠随机分为模型组80只,正常对照组(NC)20只。模型组采用熏香烟和气管内滴注猪胰蛋白酶(PEE)法建立COPD模型大鼠,以低于NC大鼠平均体重的90%作为判断发生营养不良的标准,将COPD模型大鼠分为COPD营养不良组(NM)和COPD营养正常组(MC)。取大鼠骨骼肌(膈肌、趾长伸肌)制备石蜡切片,分别采用TUNEL法测定骨骼肌细胞凋亡率,免疫组织化学法测定骨骼肌Bc l-2、Bax表达,Im age-Pro P lus version 6.0图像系统测定Bc l-2、Bax蛋白表达强度。结果 NM大鼠膈肌、趾长伸肌的凋亡率高于NC和MC(P<0.05);NM大鼠膈肌、趾长伸肌Bc l-2的表达均低于NC和MC(P<0.05);NM膈肌、趾长伸肌的Bax表达均强于MC和NC(P<0.05)。结论 COPD模型大鼠可能存在骨骼肌细胞凋亡,Bc l-2、Bax可能参与COPD模型大鼠骨骼肌细胞凋亡。

Study on Relationship between Expressions of Bcl-2 and Bax and Skeletal Muscle Apoptosis in COPD Rats

Objective To study the relationship between expressions of Bcl-2 and Bax and skeletal muscle apoptosis in COPD rats.Methods 100 healthy male adult Wistar rats were randomly divided into two groups including model group(n=80) and normal control group(NC,n=20).COPD model rats were copied by tabocco smoke inhalation and intracheally given PEE successfully.COPD model rats were redivided into two groups including malnutrition COPD group(MC)which was lower than 90% of the mean body weight of NC and non-malnutriti...