重组人p53腺病毒注射液(rAd/p53)治疗小鼠乳腺癌的实验研究

目的 探讨重组人p53腺病毒注射液(rAd/p53)不同用药方式对荷瘤小鼠乳腺癌的治疗效果.方法 细胞毒性实验,流式细胞术观察rAd/p53对乳腺癌细胞的体外抑制作用及促凋亡作用.建立乳腺癌小鼠模型,不同方式瘤内注射rAd/p53,观察对肿瘤的生长情况的影响,并对肿瘤组织进行免疫组化检查微血管密度(MVD).结果 rAd/p53体内外均可显著抑制乳腺癌细胞Ca761的生长,且早期连续瘤内给药效果好于隔天给药.结论 实验研究提示重组人p53腺病毒注射液治疗乳腺癌是有效的,早期连续用药疗效可能会更佳.

Experimental Study of Recombinant Adenoviral-Mediated p53 Gene Transfer in Breast Cancer of Mouse Model

Objective To explore the effect of rAd/p53 to breast cancer of the mice by different administrations.Methods The cell growth inhibition and apoptosis caused by rAd/p53 were detected by cytotoxicity experiment(MTT) and flow cytometry.The mice model bearing breast cancer was established using Ca761 breast cancer cell.And rAd/p53 were injected into the tumor by different administrations.The growths of tumors was observed,and detected by MVD.Results rAd/p53 could inhibit the growth of breast cancer in vitro and vivo.And the therapeutic effect of early continuous administration was better than quaque die alterna.Conclusions It is feasible for rAd/p53 to treat breast cancer,and early continuous administration may be better than other method.