不同浓度肝细胞生长因子和成纤维细胞生长因子对大鼠肝干细胞的增殖调控

目的：初步探讨不同浓度的肝细胞生长因子(hepatocyte growth factor，HGF)和成纤维细胞生长因子(fibroblast growth factor, FGF)对大鼠肝干细胞增殖的调控作用。方法：先将大鼠肝胎细胞分别种植A、B培养板上进行肝细胞的体外培养。A组：培养液中含有HGF,浓度分为别1，5，10，20，40，80及100 ng/mL。B组：培养液中含有FGF,浓度分别为1,5,10,20,40,80及100 ng/mL。对照组不加细胞因子。由A、B两组剂量效应获得HGF和FGF的最佳浓度。再将细胞接种于C组培养板进行培养,培养液中含有最佳浓度HGF联合上述各浓度FGF, 并用四甲基偶氮唑盐比色法(mononuclear cell direct cytotoxicity assay, MTT)分别测两组的光密度值（optical delnsity, OD），检测出HGF与FGF的最佳浓度组合。结果：HGF和FGF浓度与肝干细胞增殖效应呈剂量依赖性（dose-dependent）。当HGF浓度为20 ng/mL，FGF浓度为10 ng/mL时，为最佳浓度组合。结论：HGF和FGF均能对肝干细胞的增殖起促进作用,并且两者有一定协同作用。

Different concentrations of hepatocyte growth factor and fibroblast growth factor in the regulation of hepatic stem cells in rats

Objective To evaluate effect of different concentrations of hepatocyte growth factor (HGF) and fibroblast growth factor (FGF) on the regulation of hepatic stem cell proliferation.Methods Hepatic stem cells from rats were cultured under different conditions (Group A and B). HGF was used in Group A, with the concentrations of 1,5,10,20,40,80, and 100 ng/mL. Cytokines was not used in the control group. FGF was used in Group B, with the concentrations of 1,5,10,20,40,80, and 100 ng/mL. The optimal concentration...