成纤维细胞与结肠癌细胞相互作用促进细胞外基质金属蛋白酶诱导因子的表达

目的研究成纤维细胞与结肠癌细胞相互作用对二者表达细胞外基质金属蛋白酶诱导因子（EMMPRIN）的影响,初步探讨肿瘤-基质相互作用在结肠癌侵袭转移中的作用。方法结肠癌SW480细胞与HELF成纤维细胞以RPMI1640培养液分别共培养0、12、24、48h,通过RT-PCR和免疫细胞化学方法检测SW480和HELF细胞中EMMPRIN的表达。结果共培养组SW480细胞EMMPRINmRNA和蛋白的表达均明显升高,HELF细胞本来不表达EMMPRIN,与SW480细胞共培养12、24、48h后检测到EMMPRIN表达,且随时间延长而表达增加。结论成纤维细胞与结肠癌细胞相互作用上调SW480细胞EMMPRIN的表达,并诱导HELF细胞表达EMMPRIN,有可能在结肠癌侵袭转移中发挥重要作用。

Increased expression of extracellular matrix metalloproteinase inducer through interaction between fibroblasts and colonic cancer cells

OBJECTIVE: To investigate the expression of extracellular matrix metalloproteinase inducer gene (EMMPRIN) regulated by the interactions between fibroblasts and colon cancer cells, and to study its role in the invasion and metastasis of colon cancer. METHODS: Colon cancer cells (SW480) were co-cultured with fibroblasts (HELF) in RPMI 1640 media for 0, 12, 24 and 48 hours. The expression of EMMPRIN in SW480 cells and HELF cells was documented by RT-PCR and immunocytochemistry. RESULTS: The mRNA and protein expressions of EMMPRIN in SW480 cells were remarkably increased by the co-culturing with HELF cells. Although without the endogenous expression, HELF cells began to express EMMPRIN in a time-dependent manner after being co-cultured with SW480 cells. CONCLUSIONS: Intercellular interactions between colon cancer cells and fibroblasts not only up-regulate the EMMPRIN expression in SW480 cells, but also induce its expression in HELF cells. Such interactions may play a crucial role in the invasion and metastasis of colon cancer.