基于GC-MS技术研究炮制对白术芍药散中挥发性成分的影响

目的 采用GC-MS技术针对白术芍药散挥发性成分组成进行鉴别分析，同时与药材未经炮制即配伍入药的情况进行比较，探讨药物炮制过程对白术芍药散中挥发性成分的影响。方法 利用水蒸气蒸馏法提取白术芍药散的挥发油，稀释后经GC-MS进行成分分析鉴别，结合NIST 11数据库和Wiley谱库对样品中成分匹配定性，并利用归一化法计算各成分相对含量。结果 经数据整理分析得炮制前后样品同时匹配出145种成分，其中萜烯烃及含氧萜烯类化合物居多，炮制后消失的成分15种，新出现成分17种。结论 饮片是否经过炮制后配伍入药对于白术芍药散挥发性成分的组成具有一定影响，古籍中所载白术芍药散应采用炮制品进行组方用药具有现实的客观原因与深刻的科学内涵。 

Influence of Processing on Volatile Components of Baizhu Shaoyao San Based on GC-MS Technique

OBJECTIVE To identify and analyze the chemical composition of the volatile components in Baizhu Shaoyao San (BZSYS)， decoction sample composed with unprocessed herbal drugs was tested for a purpose of comparation. METHODS The volatile oil was extracted from BZSYS decoction by steam distillation， diluted and then separated by GC-MS. Components were matched and identified based on NIST 11 and Wiley databases. The relative contents of these components were calculated by normalization method. Finally， the volatile oil samples obtained from processed or unprocessed drugs were compared and analyzed. RESULTS A total of 145 components were matched by data analysis in both groups. After processing， 15 components were found to be disappeared， while 17 new components were found to be appeared. CONCLUSION The processing of herbal drugs shows a significant influence on the volatile components of BZSYS. It is a realistic and objective reason that BZSYS should be composed with processed drugs just as recorded in the ancient literature.