人骨髓基质细胞支持脐血造血细胞扩增后脐血归巢相关特性变化的研究

目的探讨人骨髓基质细胞（HBMSC）联合细胞因子对脐血（CB）单个核细胞（MNC）体外培养后造血细胞归巢相关特性的变化以评价HBMSC及细胞因子支持的体外扩增对脐血归巢相关功能的影响。方法将从新鲜CB标本中分离出的MNC分别接种于已建立的无血清培养体系：A组：对照组；B组：单用HBMSC支持；C组：单用细胞因子支持；D组：细胞因子和HBMSC联合支持。分别在0d（d0）、10d（d10）及14d（d14）用流式细胞仪检测CD34^＋CXCR4细胞、CD34^＋VLA-4^＋细胞的变化情况。结果①在体外培养过程中，各时间点D组CD34^＋CXCR4^＋细胞扩增倍数均高于A、B、C组（P〈0.05）；②B、C和D组与A组比较差异有统计学意义（P〈0．05）。结论HBMSC联合外源性细胞因子对脐血MNC进行体外培养，能有效扩增具归巢能力的造血干祖细胞数目。

Homing related activities of cord blood stem/progenitor cells after short-term ex vivo expansion supported by human bone marrow stromal cells

Objective To explore the changes of homing related activities of cord blood (CB)hemato- poietic cells during ex vivo culture supported by human bone marrow stromal cells and cytokines. Methods Mononuclear cells(MNCs) separated from CB were cultured in a serum-free with only FBMSC or only exogenous cytokines support or with both of them support culture system. On day 0、10 and 14, CD34+ CXCR+ and CD34+ VLA-4+cells were quantitated by FACS. Results After culturing, CD34+CXCR4 + cells and CD34+ VLA-4+ cells were ignificantly increased. Compared with other groups, expansion fold of CD34+ CXCR4+/CD34+ VLA- 4+cells was higher in FBMSC and cytokines supported group(P < 0. 05). Conclusion The culture system used in the study can support the expansion of hematopoietic stem and progenitor cells which had homing related ca- pacity. This culture system may be a feasible way for in vitro culture of cord blood cells.