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| 抑制活化素受体样激酶5对急性肺损伤后细胞外基质代谢的影响 | |
| 引用本文： | 卫伟,马兵,贾一韬,左新成,黄昌林. 抑制活化素受体样激酶5对急性肺损伤后细胞外基质代谢的影响[J]. 中国预防医学杂志, 2011, 0(3): 230-233 |
| 作者姓名： | 卫伟 马兵 贾一韬 左新成 黄昌林 |
| 作者单位： | 解放军第一五○中心医院烧伤整形科;第二军医大学长海医院烧伤科; |
| 基金项目： | 全军医药卫生科研项目(J115N037) |
| 摘 要： | 目的研究TGF-β/Smads信号转导通路对急性肺损伤后细胞外基质代谢的影响,以了解该信号转导通路在肺纤维化发生过程中的调控作用。方法用SB431542抑制活化素受体样激酶5(ALK5)来抑制TGF-β/Smads通路。24只雄性SD大鼠,随机分为对照组、烧伤组、早期处理组、后期处理组,每组6只。对照组施行假烫,其他3组大鼠造成30%TBSAⅢ度烧伤。早期处理组分别于烧伤后、1×24 h、2×24 h后腹腔注射SB431542,而后期处理组分别于3×24 h、4×24 h、5×24 h后腹腔注射SB431542,28 d后取肺组织,采用real-ti me PCR法检测MMP-2、MMP-9和TI MP-1 mRNA转录水平;用羟脯氨酸测试盒测定羟脯氨酸含量;并行肺脏病理学检查及评分。统计学处理采用SPSS13.0软件进行单因素方差分析判断组间差异,P<0.05为差异有统计学意义。结果抑制ALK5降低了MMP-2 mRNA转录水平,而早期抑制的效应更为明显;与急性期后抑制相比,早期抑制ALK5降低了MMP-9 mRNA转录水平,不利于胶原的降解;病理学观察见急性期后抑制ALK5将减轻肺纤维化程度。结论在肺部炎... |
| 关 键 词： | TGF-β Smads 烧伤 肺损伤 肺纤维化 |
Inhibition of activin receptor like kinase 5 activation affects extracellular matrix metabolism after acute lung injury |
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| WEI Wei,MA Bing,JIA Yi-tao,ZUO Xin-cheng,HUANG Chang-lin. Inhibition of activin receptor like kinase 5 activation affects extracellular matrix metabolism after acute lung injury[J]. China Preventive Medicine, 2011, 0(3): 230-233 | |
| Authors: | WEI Wei MA Bing JIA Yi-tao ZUO Xin-cheng HUANG Chang-lin |
| Affiliation: | WEI Wei,MA Bing,JIA Yi-tao,ZUO Xin-cheng,HUANG Chang-lin.Department of Burn and Phstic Surgery,the 150 Hospital of Chinese PLA,Luoyang,Henan 471031,China |
| Abstract: | Objective To investigate the potential roles of Smads signal transduction pathway in pulmonary fibrosis formation by investigating the extracellular matrix metabolism changes after acute lung injury.Methods Twenty-four SD rats were randomly divided into group of control,burn,SB431542 early treatment and SB431542 later treatment with 6 rats for each.Rats other than in the control group were burnt to prepare for third degree of 30% of total body surface area(TBSA)except for ones of group control.Rats of early... |
| Keywords: | TGF-β Smads Burns Lung injury Pulmonary fibrosis |
| 本文献已被 CNKI 维普 等数据库收录！ | |