| HPLC法测定黄芪中的黄芪甲苷含量 |
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| 引用本文： | 田丰,邓英杰.HPLC法测定黄芪中的黄芪甲苷含量[J].沈阳药科大学学报,2000,17(1):43-45. |
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| 作者姓名： | 田丰 邓英杰 |
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| 作者单位： | 沈阳药科大学药学系,沈阳,110015 |
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| 摘 要： | 用ＨＰＬＣ法测定了黄芪甲苷含量．样品从东北黄芪中提取，用色谱纯甲醇溶解并稀释，采用ＹＷＧ Ｃ１８ 柱，流动相为甲醇，在２０５ ｎｍ 下检测，灵敏度为０－１ ＡＵＦＳ，测定了黄芪甲苷的含量．平均回收率为９６－１ ％ ，ＲＳＤ 为２－１５ ％ ，结果表明，样品浓度在０－０１ ～０－２ ｍｇ／ｍＬ 之间与峰面积成良好的线性关系．
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| 关 键 词： | HPLC 黄芪甲苷 黄芪 |
| 修稿时间： | 1999-01-16 |
High-performance Liquid Chromatographic Determination of Astragaloside in Astragalus membranaceus(Fisch.)Bunge. |
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| Tian Feng,Deng Yingjie.High-performance Liquid Chromatographic Determination of Astragaloside in Astragalus membranaceus(Fisch.)Bunge.[J].Journal of Shenyang Pharmaceutical University,2000,17(1):43-45. |
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| Authors: | Tian Feng Deng Yingjie |
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| Abstract: | A sensitive method was developed to determine astragaloside by high performance liquid chromatography with the C 18 column.The mobile phase was methanol and the UV absorbency was monitored at 205 nm.This assay demonstrated that astragaloside had adequate sensitivity and selectivity to measure the concentrations of astragaloside in Astragalus membranaceus (Fisch.)Bunge. |
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| Keywords: | high performance liquid chromatography astragaloside Astragalus membranaceus (Fisch )Bunge |
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