Quantitative multi-contrast in vivo mouse imaging with polarization diversity optical coherence tomography and angiography |
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| Authors: | Destiny Hsu Ji Hoon Kwon Ringo Ng Shuichi Makita Yoshiaki Yasuno Marinko V. Sarunic Myeong Jin Ju |
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| Affiliation: | 1.Simon Fraser University, Biomedical Optics Research Group, Department of Engineering Science, Burnaby, British Columbia, Canada;2.University of Tsukuba, Computational Optics Group, Institute of Applied Physics, Japan;3.University of British Columbia, Department of Ophthalmology and Visual Sciences, Vancouver, British Columbia, Canada;4.University of British Columbia, School of Biomedical Engineering, Vancouver, British Columbia, Canada;5.co-first author |
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| Abstract: | ![]()
Retinal microvasculature and the retinal pigment epithelium (RPE) play vital roles in maintaining the health and metabolic activity of the eye. Visualization of these retina structures is essential for pre-clinical studies of vision-robbing diseases, such as age-related macular degeneration (AMD). We have developed a quantitative multi-contrast polarization diversity OCT and angiography (QMC-PD-OCTA) system for imaging and visualizing pigment in the RPE using degree of polarization uniformity (DOPU), along with flow in the retinal capillaries using OCT angiography (OCTA). An adaptive DOPU averaging kernel was developed to increase quantifiable values from visual data, and QMC en face images permit simultaneous visualization of vessel location, depth, melanin region thickness, and mean DOPU values, allowing rapid identification and differentiation of disease symptoms. The retina of five different mice strains were measured in vivo, with results demonstrating potential for pre-clinical studies of retinal disorders. |
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