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| 受体酪氨酸激酶抑制剂对兔OA模型软骨细胞NO的表达及软骨组织形态的影响 | |
| 引用本文： | 潘哲尔,陈乃钱,张纯武,李驰,黄小敬,叶澄宇. 受体酪氨酸激酶抑制剂对兔OA模型软骨细胞NO的表达及软骨组织形态的影响[J]. 中医正骨, 2011, 23(5): 19-23 |
| 作者姓名： | 潘哲尔 陈乃钱 张纯武 李驰 黄小敬 叶澄宇 |
| 作者单位： | 温州医学院附属第一医院,浙江,温州,325000 |
| 基金项目： | 温州市科技局科学研究基金 |
| 摘 要： | 目的：探讨受体酪氨酸激酶抑制剂genistein对兔OA模型软骨细胞NO的表达及软骨组织形态的影响,并探讨其可能的作用机制。方法：参照Hulth法建立兔骨关节炎模型,予以genistein干预后,分别按照0.1 mg.kg-1、0.2 mg.kg-1、0.3 mg.kg-1、0.4 mg.kg-1、0.5 mg.kg-1的给药剂量进行分组,行手术侧关节腔内注射,通过测定各组亚硝酸盐含量检测关节腔内NO的表达水平,同时进行软骨组织形态学检查。结果：0.3 mg.kg-1、0.4 mg.kg-1、0.5 mg.kg-1浓度genistein干预组关节腔内NO的表达水平明显低于对照组,差异具有统计学意义（P〈0.05）;对软骨组织行H.E/SOFG染色显示,在造模后第4周、第8周与第12周时,genistein干预组与对照组相比,组织损伤均明显减轻,Mankin评分均明显下降,差异具有统计学意义（P〈0.05）。结论：受体酪氨酸激酶抑制剂genistein在体内能减少骨关节炎软骨细胞NO的表达,对于延迟关节软骨在组织形态水平的退变是有效的,但是尚不能最终阻止软骨退变发生发展的进程。 |
| 关 键 词： | 骨关节炎 软骨细胞 受体酪氨酸激酶抑制剂 一氧化氮 |
The effects of genistein on the exPression of NO and ultra-microstructure of osteoarthritis cartilage and chondrocyte in OA rats |
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| PAN Zhe-er,CHEN Nai-qian,ZHANG Chun-wu,et al.. The effects of genistein on the exPression of NO and ultra-microstructure of osteoarthritis cartilage and chondrocyte in OA rats[J]. The Journal of Traditional Chinese Orthopedics and Traumatology, 2011, 23(5): 19-23 | |
| Authors: | PAN Zhe-er CHEN Nai-qian ZHANG Chun-wu et al. |
| Affiliation: | PAN Zhe-er*,CHEN Nai-qian,ZHANG Chun-wu,et al.*The First Affilatet Hospieal of Wenzhou Medical College,Ruian 325000,Zhejiang,China |
| Abstract: | Objective:To investigate the effects of recePtor tyrosine kinase inhibitor genistein on the exPression of NO and ultra-microstructure of osteoarthritis cartilage and chondrocyte in OA rats,thus try to exPress the Probable mechanisms involved.Methods:According to Hulth method,we built rat OA model and the exPression levels of NO was detected by Griess method and the change of ultra-microstructure of cartilage was observed.Results:ComPared with control grouP,less levels of NO were detected after the intervent... |
| Keywords: | osteoarthritis chondrocyte recePtor tyrosine kinase inhibitor nitric oxide |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |