升降散对急性肺损伤大鼠肺细支气管粘膜上皮细胞ICAM-1表达的影响

目的:探讨升降散对急性肺损伤模型大鼠细气管粘膜上皮ICAM-1表达的影响。方法:40只雄性SD大鼠,随机分为正常组、模型组、升降散大剂量组、中剂量组及小剂量组。给药6天后,舌下静脉注射LPS复制急性肺损伤模型,取肺组织切片,免疫组化检测。采集图像,分析细支气管粘膜上皮细胞胞质的平均灰度。结果:药物治疗组与模型组比较,能明显抑制肺细气管粘膜上皮细胞胞质ICAM-1的表达,有显著性差异。结论:升降散可通过抑制肺细气管粘膜上皮细胞胞质ICAM-1的表达,抑制炎症反应对肺组织造成的急性损伤,对急性肺损伤起到防治作用。

Effect of Shengjiang Powder on Expression of ICAM-1 of Lung Bronchiolar Epithelium Mucosae in Acute Lung Injury Rats

Objective:To investigate the effect of Shengjiang Powder on expression of ICAM-1 of lung bronchiolar epithelium mucosae in acute lung injury rats.Methods:Totally 40 healthy SD male rats of which were divided into 5 groups by random:control group,model group,Shengjiang powder high-,low-and middle-dose groups.At the 6th day after administration,reproduce acute lung injury rat models by injecting LPS into sublingual vein.Detected the expression of ICAM-1 by assessing lung histological sections by immmunohistochemistry.Capture the images and measured the average gray values of cytoplasm of lung bronchiolar epithelium mucosae.Results:Compared with model group,Shengjiang Powder could depress the expression of ICAM-1 in the cytoplasm of lung bronchiolar epithelium mucosae,statistical difference existed between treating groups and model group.Conclusion:Shengjiang Powder can preventing and treating acute lung injury by inhibiting inflammatory damage on lung tissues through depressing the expression of ICAM-1 in the cytoplasm of lung bronchiolar epithelium mucosae.