生育男性和异常精子症病人精浆一氧化氮水平的差异

目的 :通过研究精子正常和异常男性精浆中一氧化氮 (NO)含量及动态的差异 ,以了解NO与男性生育力的关系。　方法 :采用硝酸还原酶法 ,经Greiss试剂显色后 ,用分光光度法测定 174例生育男性和 2 17例异常精子症男性精浆中NO的含量。　结果 :① 174例精子生育男性精浆中均检测出NO ,平均含量为 (2 7.78± 5 .81) μmol/L,并随年龄增加而增高 ,40岁以后增高显著。 2 0～ 2 9岁组 [(2 6 .2 5± 5 .5 2 ) μmol/L]与 30～ 39岁组 [(2 8.11± 5 .87)μmol/L]相比 ,差异无显著性 (P >0 .0 5 ) ,但 40～ 49岁组 [(30 .17± 6 .14) μmol/L]与 2 0～ 2 9岁组相比 ,增高有统计学意义 (P <0 .0 5 )。② 9类精子异常症男性的精浆中NO含量均显著高于精子正常组。增高幅度以精子单项异常为最小 ,以精子的 3项异常为最高 ,2项精子异常的精浆中NO水平介于两者之间。　结论 :男性精浆中适度的NO可能具有调节精子发生的作用。

Different Levels of Nitric Oxide in Seminal Plasma of Fertile and Abnormospermic Men

OBJECTIVES: To understand the relation between nitric oxide (NO) level in seminal plasma and male fertility. METHODS: The levels of NO in seminal plasma of 174 fertile males and 217 abnormospermia patients were measured by using the reductase of nitric acid, Greiss reagent and spectrophotometry. RESULTS: 1. NO was found in all 174 samples (20-49 years) of fertile males which was (27.78 +/- 5.81) mumol/L. The NO level in seminal plasma in fertile males was became higher after age 40, and there was no significant difference between 20-29-year-old [(26.25 +/- 5.52) mumol/L] and 30-39-year-old[(28.11 +/- 5.87) mumol/L]. But the group of 40-49-year-old[(30.17 +/- 6.14) mumol/L] had a higher level of NO in seminal plasma than 20-29-year-old (P < 0.05). 2. The seminal plasma samples from nine types of abnormospermia were measured, which all had a higher level of NO than fertile males. In abnormospermia, the level of NO in seminal plasma of the patients with single abnormality increased slightly, two abnormality obviously increased, and the highest level of NO in seminal plasma appeared in three abnormality. CONCLUSIONS: This results confirmed that proper level of NO in seminal plasma may regulate the spermatogenesis.