广东省韶关地区戊型肝炎病毒基因型分析

目的了解韶关地区流行的戊型肝炎病毒基因型,并探讨戊型肝炎是否属于人兽共患病。方法通过同源比对HEV四种基因型的戊型肝炎病毒序列,选取其保守序列部分设计两对巢式聚合酶链式反应的简并引物,建立一种可同时扩增戊型肝炎四个基因型病毒RNA的PCR快速检测方法。在广东省韶关地区采集猪粪便标本和非甲非乙型肝炎病人血清,提取病毒RNA,然后进行PCR检测,阳性时对扩增片段进行基因序列测定,并进行基因型分析。结果RT-nPCR检测结果显示99份猪粪便标本中有6份HEVRNA阳性,7份病人血清样本中1份阳性,序列分析显示全部为HEV基因IV型,4份猪标本中的HEV基因序列与GenBank中人HEV的基因序列最相似,同源性为88%~94%,且其中1份猪标本中的HEV与1份病人标本中HEV的序列同源性高达91.5%。结论广东韶关地区HEV流行株为基因IV型,而且猪HEV基因序列与人HEV之间有着高度的相似性,结果证实戊型肝炎是一种人兽共患传染病。

Phylogenetic analysis of hepatitis E virus (HEV) in fecal samples from pigs and in serum from human in Shaoguan ,Guangdong province

ObjectiveTo investigate the genotyping of HEV in Shaoguan, Guangeong province. MethodsTwo pairs of consensus primers were designed according to the conserved sequence region from four-genotype HEV strains for nested PCR. HEV RNA in pig fecal sample and in serum from patient with non-A, non-B hepatitis was detected by nested PCR. Positive PCR product was sequenced and analyzed phylogenetically with neighbor-joining method. ResultsHEV RNA was detected in 6 of the 99 pig fecal samples and 1 of the 7 patient sera. Sequence analysis show that all of the positive isolates were genotype IV. ConclusionThese findings suggest that HEV genotype IV is the endemic strain in Shaoguan and hepatitis E is a zoonosis.