三乙酰莽草酸对血小板聚集的抑制作用

目的：研究三乙酰莽草酸（TSA）对血小板聚集功能的抑制作用及其作用机理。方法：用比浊法测定血小板聚集功能，分光光度法测定MDA的含量，放免法测定TXB₂，6-酮-PGF_1α，cAMP和cGMP的含量。结果：TSA 12.5，25，50，100和200 mg.kg^-1 ig明显抑制ADP和胶原诱导的大鼠血小板聚集；TSA 12.5，50和200　mg.kg^-1 ig显著增加大鼠血小板内cAMP水平，但不影响cGMP水平。TSA 200 mg.kg^-1对AA诱导的血小板中MDA的生成，ADP诱导的血小板中TXB2和腹主动脉壁6-酮-PGF_1α的生成有轻度抑制作用。结论：TSA抑制血小板聚集作用部分与血小板内cAMP水平升高有关。

INHIBITORY EFFECTS OF TRIACETYLSHIKIMIC ACID ON PLATELET AGGREGATION

AIM: To study the inhibitory effects of triacetylshikimic acid (TSA) on rat platelet aggregation and its mechanism. METHODS: Light transmission was used to measure platelet aggregation. Methods of spectrophotometry and radioimmunoassay were used to measure the levels of malondialdehyde(MDA), thromboxane B2(TXB2), 6ketoPGF1, cAMP and cGMP. RESULTS: TSA 125, 25,50,100 and 200 mgkg-1d-1 ig for 3 d inhibited the rat platelet aggregation induced by ADP and collagen. TSA 125, 50 and 200 mgkg-1 markedly increased the cAMP level and exhibited no effect on the cGMP level in rat platelets. TSA 200 mgkg-1 was shown to slightly inhibit AAinduced MDA generation and ADPinduced TXB2 formation in rat platelets, and to slightly suppress 6ketoPGF1 generation from the abdominal aortae. CONCLUSION: TSA was found to be a potent inhibitor of platelet aggregation, which was partially concerned with the elevation of cAMP in platelets.