髓过氧化物酶、CD11b和IL-8在哮喘大鼠模型中表达增加

目的观察哮喘模型大鼠中性粒细胞(PMN)CD11bI、L-8和髓过氧化物酶(MPO)的表达,探讨PMN在哮喘中的作用及其可能的机制。方法复制哮喘大鼠模型,随机分成哮喘组和对照组,分离纯化血PMN;免疫组化法检测MPO表达,ELISA法测定IL-8蛋白,流式细胞术测定血PMN CD11b表达,支气管肺泡灌洗液(BALF)行细胞计数。结果哮喘组肺组织和血PMN中MPO、CD11b及血PMN和BALF中IL-8的水平显著高于对照组(P<0.01)。哮喘组BALF中PMN和嗜酸性粒细胞计数显著高于对照组(P<0.01)。结论BALF中PMN数量增加及血中PMN和肺组织中CD11bI、L-8和MPO在哮喘时表达增加,他们可能参与了哮喘的炎症过程。

Increased expression of myeloperoxidase, CD1 1b and IL-8 in rat asthma model

Objective To observe expression of myeloperoxidase(MPO),CD11b and IL-8 and to studay the functions and possible mechanism of polymorphonuclear leukocyte(PMN) in rat asthma.Methods The rats were randomly divided into two groups.Blood PMN were isolated and purified,MPO were detected by immunohistochemistry techniques,IL-8 was detected by ELISA,blood PMN CD11b were detected by flow cytometry(MFI).The total cell numbers and differential cell numbers in BALF were counted.Results MPO in bronchial wall and blood PMN,CD11b and IL-8 in blood and BALF expressions in asthma group were significantly increased than those in control group(P<0.01).Number of PMN and eosinophil in BALF in asthma group was significantly higher than that in control group(P<0.01,respectively).Conclusion Expressions of MPO,CD11b,IL-8 and PMN significantly increase in asthma,they may be involved in inflammation of asthma.Increased levels of CD11b,IL-8,and MPO may be responsible for function of PMN.