BioAggregate和MTA对体内破骨细胞分化和功能的影响

目的研究口腔生物陶瓷材料Bio Aggregate及MTA对体内LPS诱导的炎性骨吸收的影响。方法 6周龄C57/BL6雄性小鼠随机分为四组:PBS组、LPS组、Bio Aggregate+LPS组和MTA+LPS组,每组6只。LPS干预小鼠7 d后,取出颅盖骨进行显微-CT扫描、HE染色、酶组织化学染色和组织免疫荧光染色,观察骨破坏面积、破骨细胞的形成以及组织蛋白酶K(cathepsin K)的表达。结果 Bio Aggregate和MTA浸提液明显减少LPS诱导的破骨细胞形成和小鼠颅盖骨炎性骨破坏。与破骨细胞功能密切相关的组织蛋白酶K的表达在Bio Aggregate和MTA浸提液的作用下被显著下调。结论新型口腔生物陶瓷材料Bio Aggregate和传统材料MTA能够抑制体内炎性骨吸收。

Effect of BioAggregate and MTA on osteoclast differentiation and function in vivo

Objective To investigate the effect of BioAggregate and MTA on LPS?induced bone destruction in vivo, and to compare its performance with that of mineral trioxide aggregate. Methods Six?week?old C57/BL6 male mice were divided into the injection group containing 10 mg/kg LPS with or without the material extracts ( n=6) and the vehicle?injection group with the culture medium a?lone ( n=6) . After LPS stimulation for 7 days, mice were sacrificed and calvarial bones were prepared for micro?computed tomography scanning, histologic analysis and double immunofluorescence staining. The bone damage area, osteoclast formation, and cathepsin K expression were observed. Results BioAggregate and MTA extracts markedly reduced osteoclast numbers and attenuated bone resorp?tion in LPS?challenged mouse calvaria. The expression level of osteoclastogenic cathepsin K was also decreased by BioAggregate and MTA extracts. Conclusion BioAggregate and MTA show comparable inhibitory effects on osteoclast differentiation and inflammatory bone resorption in vivo.