P LGA／P CL混纺技术改良P LGA电纺膜收缩性能的初步研究

目的：利用聚乳酸－聚乙醇酸共聚物／聚己内酯（ PLGA ／ PCL）混纺技术在不影响纯PLGA静电纺丝膜生物相容性的前提下改良其遇水收缩的缺陷，以使其操作性能更接近临床实际应用。方法：将不同重量比的PLGA ／ PCL（10∶0、7∶3、6∶4、5∶5及0∶10）混合，利用静电纺丝技术制得电纺膜，表征比较纺丝直径及收缩性。在相同条件下在膜上培养成骨细胞，比较不同电纺膜的细胞相容性，并在扫描电镜下观察不同电纺膜表面形貌及细胞粘附形态。结果：随PCL比例增加，纺丝直径有所增加，但在扫描电镜下不同电纺膜表面形貌及细胞粘附形态并无明显差异；加入PCL后混纺膜的细胞相容性较纯PLGA略有下降，但仍比纯PCL高，且不同比例的PLGA／PCL（7∶3、6∶4、5∶5）混合电纺膜细胞相容性无统计学差异；纯PLGA膜呈现极大的收缩比率，随PCL的添加收缩比率明显下降，且不同的添加比例组间无明显差异。结论：在PLGA中添加一定比例的PCL可以有效改善纯PLGA膜的收缩性能，且PCL的加入对PLGA良好的生物相容性影响较小。

Preliminary study of ameliorating the dimensional shrinkage of PLGA electrospun membrane by blending PLGA and PCL

Objective:To tackle the dimensional shrinkage of electrospun membrane of ( poly( lactic?co?glycolic acid) , PLGA) by adding a certain amount of ( poly(ε?caprolactone) , PCL) in order to make it qualify for clinical use with the biocompatibility of PLGA not significantly affected. Methods:We blended PLGA and PCL in different weight ratio (10 ∶ 0, 7 ∶ 3, 6 ∶ 4, 5 ∶ 5 and 0 ∶ 10), and fibrous membranes were collected by electrospun. The diameters and shrinkage of the fibers were compared. The biocompatibilities of different membranes were compared by culturing osteoblasts under the same condition on them. The topography of the membranes and the morphology of the cells adherent on different membranes were observed with scanning electron microscope. Results:The diameter of electrospun fibers rose as the ratio of PCL increased, but the topography of the membranes and the morphology of the cells adherent on them presented no significant diversity among different PLGA/PCL ratio. The biocompatibility of PLGA declined with the adding of PCL, and different blending groups (7 ∶ 3, 6 ∶ 4, 5 ∶ 5) showed no statistical differences, but they were still better than PCL. PLGA electrospun membranes showed significant shrinkage in phosphate buffer solution, and the dimensional shrinkage decreased with the ad?dition of PCL, but the decrease presented no significant differences among different PLGA/PCL ratio. Conclusions:Blending PLGA/PCL at a certain ratio can prominently ameliorate the shrinkage of PLGA, furthermore, the blending has little influence on the excellent biocompatibility of PLGA.