荧光标记法对“脑回形”微弧氧化表面种植体早期骨结合的评价

目的:本文将通过双荧光标记法在兔股骨髁部植入具有“脑回形”(cortex-like)形貌的微弧氧化二氧化钛(TiO2)膜层的种植体,来评价的骨-种植体结合的能力.方法:实验组MAO(cortex-like)为“脑回形”形貌种植体,对照组SLA为具有同等规格的SLA形貌的种植体,共计36颗,通过扫描电镜(SEM)等对材料表面进行表征,随机植入18只新西兰雄性兔的股骨髁部,分别于4w和8w处死取样,处死前第13、14d颈部皮下注射茜素红,处死前第3、4d颈部皮下注射钙黄绿素,通过茜素红-钙黄绿素双色标记示踪检测其矿化速率MAR(mineralization apposition rate).结果:类“脑回形”形貌氧化层由微米级沟槽和纳米级孔洞组成,属超亲水性种植体.4w时间段,实验组MAO组矿化速率MAR(mineralization apposition rate)高于SLA组,但差异无统计学意义(P＞0.05),第8w,MAO与SLA组的MAR值相应的均有所增加,但差异无统计学意义,结论:“脑回形”(cortex-like)形貌的二氧化钛、具有双微层结构膜层,形成与SLA一致的骨结合效果,能早期促进种植体的骨结合,是一种良好的种植体改性方法.

Animal studies of osseointegration of a porous TiO2 coatings with cortex-like slots formed on Ti by micro-arc oxidation treatment

Objective:This study aimed to investigate the effects of MAO treatment with cortex-like coating on osseointegration in rabbit femoral condyle compared with the SLA coatings Ti implants by double fluorescence labeling method.Methods:The cortex-like morphology Ti surface was produced by MAO,The SLA surface was used as a contrast.The surface characteristics were evaluated by scanning electron microscope (SEM) and contact Angle.Thirty-six threaded implants (18MAO implants and 18SLA implants) were placed in the male femoral condyles of 18 New Zealand White rabbits.For the double fluorescence labeling stain,alizarin red and calcein were subcutaneously injected into the rabbits 13,14 days and 3,4 days before the sacrifice.Double fluorescence labeling method were performed at 4 weeks and 8weeks after implantation.Results:Morphology microstmcture of MAO cortex-like is composed of microscale and nanoscale structure,which have better wettability,there are no differences at 4 and 8 weeks on MAR.The value of MAR is corresponding increased.Conclusion:These promising results indicated that cortex-like coating of Ti surface improved implant osseointegration in rabbit femoral condyle,and formed in accordance with the SLA in osseointegration and has good biocompatibility and will become a good implant modification methods.