骨髓间质干细胞心肌分化过程中细胞内钙离子浓度的变化

目的研究骨髓间质干细胞心肌分化前、后钙离子浓度变化。方法用5-杂氮胞苷(5-azacytidine)体外诱导猪骨髓间质干细胞使之向心肌分化；ELASA法测定分化前、后细胞内心肌肌钙蛋白Ⅰ(cTnⅠ)变化；应用离子图像分析系统测定分化前、后细胞内钙离子浓度变化。结果(1)经5-杂氮胞苷诱导后细胞形态发生变化；(2)诱导后第3周起细胞内cTnⅠ明显增高；(3)诱导组细胞内钙离子浓度较对照组高，且诱导前后细胞内钙释放机制不同。结论骨髓间质干细胞体外经5-杂氮胞苷诱导后可具有心肌细胞的某些特性，这一过程与钙信号有关。

Change of calcium concentration in bone marrow mesenchymal stem cell-derived cardiac myocytes

Objective To investigate the change of calcium concentration during the differentitation of porcine bone marrow mesenchymal stem cells. Methods Porcine bone marrow mesenchymal stem cells were induced by 5-azacytidine in vitro, the expression of cardiac troponin I (cTnI) was identified by ELASA method. Ion imaging system was used to analyse intracellular calcium concentration. Result (1)The morphology of stem cells changed after induced by 5-azacytidine; (2)The intracellular cTnI increased after three weeks induced by 5-azacytidine; (3)The intracellular calcium concentration of differential group is higher than that of control group. Conclusion Bone marrow mesenchymal stem cells posses some characteristics of cardiac myocytes after induced by 5-azacytidine in vitro, which may be related to calcium signal.