| 同型半胱氨酸对血管内皮细胞PAI-1活性及其mRNA水平的影响 |
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| 引用本文： | 方理刚,朱文玲,朱广瑾. 同型半胱氨酸对血管内皮细胞PAI-1活性及其mRNA水平的影响[J]. 基础医学与临床, 2002, 22(1): 67-69 |
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| 作者姓名： | 方理刚 朱文玲 朱广瑾 |
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| 作者单位： | 中国医学科学院,中国协和医科大学,北京协和医院,心内科,北京,100730;中国医学科学院,中国协和医科大学,基础医学研究所,北京,100005 |
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| 摘 要： | 探讨血浆同型半胱氨酸（Hcy）致血管病变的机制。培养人脐静脉内皮细胞株,用发色底物法测定细胞上清的纤溶酶原激活物抑制物－1（PAI－1）活性,细胞原位杂交及辉度扫描检测PAI－1 mRNA水平。结果表明H 驻作用血管内皮细胞后,PAI－1活性随Hcy浓度增加和Hcy作用时间延长而呈递增趋势,PAI－1 mRNA灰度面积积分值随Hyc浓度的增加而增加。提示Hcy抑制内皮细胞纤溶能力是Hcy致血栓形成和血管病变的一个机制。
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| 关 键 词： | 同型半胱氨酸 内皮细胞 纤溶酶原激活物抑制物-1 |
| 文章编号： | 1001-6325(2002)01-0067-04 |
| 修稿时间： | 2001-05-25 |
Effect of homocysteine on the PAI-1 activity and mRNA expression in vascular endothelial cells |
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| FANG Li gang,ZHU Wen ling,ZHU Guang jin. Effect of homocysteine on the PAI-1 activity and mRNA expression in vascular endothelial cells[J]. Basic Medical Sciences and Clinics, 2002, 22(1): 67-69 |
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| Authors: | FANG Li gang ZHU Wen ling ZHU Guang jin |
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| Abstract: | To investigate the mechanism of homocysteine(Hcy) induced cardiovascular disease. Human umbilical vein endothelial cell line ECV304 cells were treated with different concentration of Hcy(0.01?0.1?1.0?5.0mmol/L), PAI 1 activity in the supernant of the cells was measured with chromogenic assay and the PAI 1 mRNA level was detected by in situ hybridization.The PAI 1 activity of endothelial cells increased by Hcy in both dose dependent and time dependent manner. The increase of the PAI 1 mRNA level accompanied with the increase of Hcy concentration.The result shows that the inhibiton of fibrinolytic system in vascular endothelial cells by Hcy is a pathophysiologic mechanism of Hcy induced cardiovascular disease. |
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| Keywords: | homocysteine endothelium plasminogen activitor inhibitor |
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