乐果对人外周血淋巴细胞热应激蛋白70表达的影响

目的研究乐果对人外周血淋巴细胞热应激蛋白70(HSP70)表达的影响,初步探讨HSP70用于有机磷农药生产的环境及生物监测的可能性。方法选取健康对照者11人及不同工种的乐果接触工人35人,分离其外周血淋巴细胞后,用流式细胞仪检测HSP70基础表达及经乐果体外诱导后水平,同时检测其全血乙酰胆碱酯酶(AChE)活力,分析其可能的影响因素。结果接触者与对照者外周血淋巴细胞HSP70基础表达水平分别为41．24%±10．45%和23．97%±4．29%,AChE活力分别为(125．23±7．97)、(145．36±8．78)U/ml,差异均有统计学意义(P＜0．01)。不同工种乐果接触工人其外周血淋巴细胞HSP70基础表达水平分别为47．34%±11．87%和38．05%±8．20%,差异有统计学意义(P＜0．05),但AChE活力差异无统计学意义(P＞0．05)。对接触者HSP70基础表达水平影响有统计学意义的因素按程度大小依次为：健康状况、环境浓度和工龄,它们共可解释HSP70至少88%的变异；对AChE活力影响有统计学意义的因素只有工龄,其只能解释AChE约12%的变异。经体外乐果诱导后,对照组HSP70诱导水平较接触组高,差异有统计学意义(P＜0．01)。不同人群淋巴细胞HSP70表达增加幅度不同,按其大小依次为：对照组、操作工和包装工。对HSP70变化率影响有统计学意义的因素为环境浓度和工龄。结论HSP70可作为综合反映乐果接触工人自身状况及所处作业环境的指标。

Effect of dimethoate on the expression of heat shock protein 70 in peripheral blood lymphocytes of human beings

OBJECTIVE: To study the effect of dimethoate on the expression of heat shock protein 70 (HSP70) in peripheral blood lymphocytes of human beings and to explore the feasibility of HSP70 in biomonitoring among workers exposed to organophosphorous pesticides. METHODS: Peripheral blood lymphocytes were isolated from subjects, comprising 11 people of the control group and 35 workers of the exposure group exposed to dimethoate. Flow cytometry was used for detecting both the basic level and the level of the dimethoate-induced expression of HSP70. The activity of whole blood acetylcholinesterase (AChE) was examined at the same time. Then the potential influential factors to HSP70 expression and AChE activity were analyzed. RESULTS: The basic level of HSP70 expression in the exposure group and the control group was 41.24% +/- 10.45% and 23.97% +/- 4.29% respectively. The activity of AChE in these two groups were (125.23 +/- 7.97) and (145.36 +/- 8.78) U/ml respectively. Both differences were statistically significant (P < 0.01). Among the exposure group, the basic level of HSP70 expression of the two categories comprising operators and packers, were 47.34% +/- 11.87% and 38.05% +/- 8.20% respectively (P < 0.05), while there was no significant difference (P > 0.05) in AChE activity between these two categories. The factors that had significant influence on the HSP70 basic level of the exposure group were the health condition, the environmental concentration of dimethoate and the exposure time in order, according to their significance of influence. At least 88% variance of HSP70 could be explained by these factors. The only factor that could influence AChE activity significantly was the exposure time, and it could only explain about 12% variance of AChE activity. After the treatment of dimethoate in vitro, the level of the induced expression of HSP70 in the control group was significantly higher than that of the exposure group (P < 0.01). The increasing order was the control group, the group of packers and the group of operators according to the increasing extent and there were significant difference among them (P < 0.01). The factors that could significantly influence the change ratio of HSP70 expression were the environmental concentration and the exposure time. CONCLUSION: HSP70 is a potential index that can reflect the individual and environmental conditions of workers exposed to dimethoate comprehensively.