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| HPLC测定水蛭仿生酶解有效部位中次黄嘌呤及尿苷 | |
| 引用本文： | 王艳,杨培民,代龙,刘国飞.HPLC测定水蛭仿生酶解有效部位中次黄嘌呤及尿苷[J].中国实验方剂学杂志,2011,17(13):74-76. |
| 作者姓名： | 王艳 杨培民 代龙 刘国飞 |
| 作者单位： | 1. 山东中医药大学,济南,250355 2. 山东中医药大学附属医院,济南,250011 3. 北京和润创新医药科技发展有限公司,北京,100021 |
| 基金项目： | 科技部"重大新药创制"专项 |
| 摘 要： | 目的:测定水蛭仿生酶解有效部位中次黄嘌呤及尿苷的含量,为其质量控制提供科学依据。方法:采用SinoChrom ODS-BP液相色谱柱,以甲醇-水为流动相,测定波长254 nm。结果:次黄嘌呤在0.88～14.08 g·L-1呈良好线性关系(r=0.9997),平均加样回收率为99.69%,RSD 1.23%;尿苷在2.12～33.92 g·L-1呈良好线性关系(r=0.999 8),平均加样回收率为99.20%,RSD 1.17%。结论:该法操作简便,结果可靠,为水蛭仿生酶解有效部位的质量研究提供有效的方法。 |
| 关 键 词： | 水蛭 次黄嘌呤 尿苷 高效液相色谱 |
| 收稿时间： | 2011/1/18 0:00:00 |
Determination of Hypoxanthine and Uridine in Leech by HPLC |
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| WANG Yan,YANG Pei-min,DAI Long and LIU Guo-fei.Determination of Hypoxanthine and Uridine in Leech by HPLC[J].China Journal of Experimental Traditional Medical Formulae,2011,17(13):74-76. | |
| Authors: | WANG Yan YANG Pei-min DAI Long and LIU Guo-fei |
| Institution: | Shandong University of Traditional Chinese Medicine, Jinan 250355, China;The Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250011, China;Shandong University of Traditional Chinese Medicine, Jinan 250355, China;Beijing He-Run Innovative Medical Technology Development Co., Ltd, Beijing 100021, China |
| Abstract: | Objective: To determine hypoxanthine and uridine in leech to provide scientific basis for its quality control. Method: An ODS-BP liquid chromatographic column was used.The mobile phase was methanol-water.Wavelength was at 254 nm. Result: The linear range of hypoxanthine was within 0.88-14.08g ·L-1(r=0.999 7). The sample recovery rate was 99.69%,RSD 1.23%.The linear range of uridine was within 2.12-33.92 g ·L-1(r=0.999 8).The sample recovery rate was 99.20%,RSD 1.17%. Conclusion: This method has good repeatability and flexibility.It can be used for quality control in production of leech. |
| Keywords: | leech hypoxanthine uridine HPLC |
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