| 人IgG1FccDNA的克隆与鉴定 |
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| 引用本文： | 安云庆,柯岩,陈金栋.人IgG1FccDNA的克隆与鉴定[J].首都医学院学报,2000,21(2):93-96. |
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| 作者姓名： | 安云庆 柯岩 陈金栋 |
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| 作者单位： | 首都医科大学微生物学和免疫学教研室 |
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| 基金项目： | 国家自然科学基金资助项目 !(395 70 6 6 6 ) |
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| 摘 要： | 根据编码人IgG1Fc(Fcγ1 )的基因序列 ,设计合成 1对与之相匹配的引物。采用RT PCR法 ,从正常人白细胞mRNA中扩增获得预期目的基因Fcγ1 70 0bp基因片段 ,成功构建PUC1 8 Fcγ1 70 0 重组克隆载体 ,酶切、酶谱分析与预期结果相符 ;DNA测序结果与GenBank报道一致。
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| 关 键 词： | I_gG1Fc(Fcγ1) PUC18-Fcγ1_(700)重组克隆载体 RT-PCR |
| 收稿时间： | 1999-08-27 |
Cloning and Identification of Human IgG1Fc cDNA |
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| An Yunqing,Ke Yan,Chen Jindong.Cloning and Identification of Human IgG1Fc cDNA[J].Journal of Capital University of Medical Sciences,2000,21(2):93-96. |
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| Authors: | An Yunqing Ke Yan Chen Jindong |
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| Institution: | Department of Microbiology and Immunology, Capital University of Medical Sciences |
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| Abstract: | According to the cDNA sequences which encode the human IgG1Fc, a pair of primers were designed; The expected gene (Fcγ1700bp) which encode Fcγ1 was amplified by RT-PCR from mRNA extracted from normal person′s leukocyte; PUC18-Fcγ1700 recombinant cloning vector was successfully constructed, and the enzyme digestion analysis are identical to expected results. Sequences are identical to those in GenBank report. |
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| Keywords: | RT-PCR |
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