ADM对大鼠早期肾脏机械损伤的影响

目的探讨外源性肾上腺髓质素（ADM）对肾脏机械性损伤早期肾小管评分的影响。方法选健康成年普通级Wistar大鼠104只,随机分为4组：正常对照组8只,创伤组32只,创伤前注射ADM组32只,创伤后注射ADM组32只。后3组动物分别于创伤后1、6、12和24?h各处死8只,采用自由落体打击仪直接打击大鼠脊肋区制作肾机械性损伤模型。两给药组分别于创伤前、后10?min腹腔注射ADM（0.1?nmol/kg）。3组肾损伤大鼠平均分为4批,分别于创伤后1、6、12和24?h迅速解剖动物提取下肾脏标本,10%甲醛固定。HE染色观察创伤后肾组织的基本病理改变。结果单纯创伤组于创伤早期（1、6?h）肾小管评分明显升高（P＜0.05）,急性创伤后期（12、24?h）上述指标逐渐降低,接近正常对照组（P＞0.05）。外源性ADM可降低肾小管评分。结论肾脏损伤期间给予外源性ADM对肾组织均有明显的保护作用,其作用强弱与给药时间关系密切。

Effects of Adrenomedullin on rats with early trauma in the kidney

Objective To investigate the effects of extragenous Adrenomedullin (ADM) on the score of the renal tubule in rats with early renal mechanical trauma. Methods 104 healthy adult plain grade Wister rats were randomly divided into four groups: 8 in the control group, 32 in the trauma group, 32 in the group treated with ADM before trauma, and 32 in the group treated with ADM after trauma. The experimental models were prepared by free dropping a ferrous hammer in the last three groups. ADM (0.1 nmol/kg) was intrapertoneally administrated 10 minutes before trauma or after trauma in the ADM groups. Renal tissues deter-mined the patho-transform changes by HE staining. Results The score of the renal tubule was distinctively increased in the early phase (1 h and 6 h) (P<0.05) but significantly decreased to the level of the control group in the late phase post trauma(12 h and 24 h) (P>0.05). Conclusion Extragenous ADM can protect the kidney, which has relations to the time of intervention.