| 用核酸分子杂交技术产前诊断人巨细胞病毒感染 |
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| 引用本文： | 陶幼慈 孙永玉 蒋哓薇. 用核酸分子杂交技术产前诊断人巨细胞病毒感染[J]. 中国优生与遗传杂志, 1995, 3(6): 15-17 |
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| 作者姓名： | 陶幼慈 孙永玉 蒋哓薇 |
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| 作者单位： | 同济医科大学附属协和医院妇产科遗传室 |
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| 摘 要： | 用ELISA法筛选早孕妇女和中孕引产妇女积压人巨细胞病毒IgM(HCMV-IgM)，早孕妇女阳性者在旱12．20周(中孕引产妇女阳性者在引产时)留羊水，用生物素标记的HCMV-DNA探针斑点杂吏检测其羊水中的HCMV-DNA。对胎儿巨细胞病毒惑染作出产前诊断。结果：早孕妇女384例．血HCMV-IgM阳性26例，此26例羊水HCMV-DNA阳性9例，其中7例出生时留脐血进一步检测HCMV-IgM和HCMV-DNA。证实有HCMV感染。中孕引产妇女125例。血HCMV-IgM阳性10例。此10例羊水HCMV-DNA阳性4例。
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| 关 键 词： | 阳性 HCMV-DNA HCMV-IgM 中孕引产 早孕 产前诊断 妇女 核酸分子杂交 探针 斑点 |
Prenatal diagnosis of human cytomegalovirus infection by nucleic acid hybridization |
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| Tao Youci,et al.. Prenatal diagnosis of human cytomegalovirus infection by nucleic acid hybridization[J]. Chinese Journal of Birth Health & Heredity, 1995, 3(6): 15-17 |
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| Authors: | Tao Youci et al. |
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| Abstract: | Human cytomegalovirus IgM (HCMV-IgM)in blood of pregnant women in the first trimester and midgestation induction was detected by ELISA.For pregnant women of HCMV-IgM positive in 12-20 weeks'gestation,amniotic fluids(10ml)were collected and HCMV-DNA in amniotic fluid was detected by dot blot hybridization with biotinlabelled probe in order to diagnose fetal HCMV infection before birth. Results: of the 384 pregnant wonen in the first trimester,26(6.77%)were positive for HCMV-IgM in blood,9 cases out of the above 26 cases were positive for HCMV-DNA in amniotic fluids,7 out of 9 umbilical cord blood were collected at delivery for further examination of HCMV-IgM and HCMV-DNA.The results were consistent with prenatal examination.Of the 125 women who made induction in the second trimester, 10 were positive for HCMV-IgM in blood. Of 10,4 were positive for HCMV-DNA in amniotic fluids. |
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| Keywords: | Nucleic acid hybridization Cytomegalovirus Prenatal diagnosis |
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