肺癌细胞与外周血淋巴细胞体外药敏实验的研究

目的探讨肺癌患者外周血淋巴细胞和癌细胞对8种临床常用化疗药物敏感性的关系，找出两者之间的相关性，指导失去手术机会的肺癌患者化疗用药的个体化。方法应用四甲基偶氮唑盐（MTT）法测定40例肺癌患者外周血淋巴细胞及癌细胞对化疗药物的敏感性，利用SPSS10．0软件计算两者之间的相关性。结果外周血淋巴细胞与肺癌细胞对8种临床常用化疗药物的敏感性有很好的相关性，差异无统计学意义（P〉0．05）。结论对失去手术机会的肺癌患者，外周血淋巴细胞可代替癌细胞行MTT体外药敏检测，对肺癌临床化疗药物的选择具有重要的参考价值。

Relative study of chemosensitivities of peripheral blood lymphocytes and cancer cells of lung cancer in vitro

Objective To evaluate the correlation of chemosensitivity of cancer cells and peripheral blood lymphocytes to 8 chemotherapy agents in vitro,which was helpful to guide clinical chemotherapy for patients with lung cancer. Methods The chemosensitivity of the peripheral blood lymphocytes and tumor cells to 8 clinical routine che-motherapeutic agents was tested in 40 cases of lung cancer by using MTT method. SPSS 10. 0 was used to analyze the above indicators. Results There was no significant difference in the sensitivity of 8 clinical routine chemotherapeutic agents between peripheral blood lymphocytes and tumor cells of patients with lung cancer(P > 0. 05) ,which was posi-tive correlated to chemotherapeutic drugs between peripheral blood lymphocytes and tumor cells. Conclusion The chemosensitive test of the peripheral blood lymphocytes with MTT method in vitro was valuable for reference of selec-ting anticancer drugs in clinic for patients with lung cancer.