旁路活化补体协同TNF-α对血管内皮细胞ICAM-1表达和粘附作用的影响

目的:探讨旁路活化补体协同TNF-α对血管内皮细胞ICAM-1表达及中性粒细胞-内皮细胞(PMN-EC)粘附的影响。方法:采用酵母多糖活化人血清(ZAHS)单独、和TNF-α协同作用于体外培养的内皮细胞单层, 用直接细胞计数和改良细胞ELISA方法检测中性粒细胞-内皮细胞粘附率的变化和细胞间粘附分子(ICAM-1)的表达。结果:旁路活化补体单独作用于内皮细胞对PMN-EC粘附和ICAM-1的表达仅有轻微影响;和40μg/L的TNF-α共同作用可以明显促进PMN-EC粘附率和ICAM-1的表达。结论:旁路活化补体对TNF-α诱导的PMN-EC粘附及ICAM-1的表达具有促进作用, 从而引起炎症反应。

Influences of bypass-activated complement and TNF-α on endothelial cells to express ICAM-1 and PMN-EC adhesion

AIM:To investigate the influences of bypass-activated complement and TNF-α on intercellular adhesion molecule 1(ICAM-1) expression in endothelial cells, and polymorphonuclear neutrophil-endothelial cell (PMN-EC) adhesion.METHODS:In vitro, zymosan-activated human serum(ZAHS)and/or TNF-Adirectly stim-ulated the HUVECS monolayers.PMN-EC adhesion percentage was measured, and modified whole-cell ELISA was used for measurement of ICAM-1.RESULTS:ZAHS alone resulted in no significant change in the degree of PMN adhesion and the level of ICAM-1. Activation of HUVECS with TNF-α followed by ZAHS stimulation resulted in greater increase in PMN-EC adhesion and ICAM-1 expression, as compared with the activation with TNF-α alone.CONCLUSION:Bypass-activated complement can promote ICAM-1 expression and neutrophil -endothelium adhesion induced by TNF-α, and so it is effective for promoting inflammation.