表皮生长因子受体EGFR和Ki67在非小细胞肺癌中的表达及其相关性研究

目的 :探讨表皮生长因子受体 (epi dermalgrowthfactorreceptor ,EGFR)和Ki67在非小细胞肺癌 (non smallcelllungcancer,NSCLC)中的表达及其与NSCLC发生、发展及预后的关系。方法 :对经随访资料完整的术后NSCLC组织标本 60例、癌旁组织 2 0例和肺正常组织 5例 ,采用SP免疫组化法检测EGFR和Ki67。对术后辅助性化疗 3 6例 ,分析疗效与EGFR和Ki67表达的关系。结果 :NSCLC中EGFR表达阳性率为 65 % ,阳性细胞的棕黄色颗粒位于细胞质 ;Ki67表达阳性率为 81 67% ,阳性细胞的棕黄色颗粒位于细胞核 ;2 0例癌旁组织及 5例正常肺组织未见阳性染色细胞。癌组织EGFR和Ki67表达与癌旁组织相比差异有统计学意义 ,P <0 0 1。NSCLC中EGFR和Ki67阳性表达与年龄、性别、吸烟与否、肿瘤细胞的病理类型差异无统计学意义 ,P >0 0 5 ;与肿瘤大小、病理分级、淋巴结转移和TNM分期密切相关 ,P <0 0 5。EGFR和Ki67表达阳性者 3年生存率分别为19 86%和 3 1 3 3 % ,低于阴性表达 70 11%和90 91% ,P <0 0 5 ;接受术后辅助性化疗的患者 ,EGFR和Ki67阳性表达复发转移率分别为 70 3 7%和 62 5 0 % ,高于阴性表达 11 11%和 0 ,P <0 0 5。结论 :EGFR和Ki67在NSCLC中的异常或过度表达与肿瘤发生、发展密切相关 ,两者表达具有协同作

Expressions of epidermal growth factor receptor (EGFR) and Ki67 in non-small cell lung cancer and correlative research

OBJECTIVE:To explore the expressions of EGFR and Ki67 in non-small cell lung cancer and the relationships between above parameters and the occurrence, progression and prognosis of NSCLC. METHODS: The expressions of EGFR and Ki67 were detected in 60 NSCLC samples, 20 lung tissues adjacent to cancer and 5 normal lung tissues as control by S-P immunohistochemical method. RESULTS: The EGFR and Ki67 positive rates were 65% and 81.67% in 60 NSCLC,respectively. The brown granules of EGFR positive cell were mainly distributed in the cytoplasm, but the brown granules of Ki67 positive cell were in the nuclei. Positive cells were not found in 20 lung tissues adjacent to cancer and 5 normal lung tissues, otherwise , there were notable difference between cancer tissues and controls,P<0.01.The EGFR and Ki67 positive expressions were not correlated to age,sex,smoking history and the pathologic types of tumor cell,P>0.05, but were significantly correlated with tumor size,the sub-grade of tumor cell,lymph node metastasis and TNM stage,P<0.05. The 3-year survival rate of EGFR and Ki67 positive patients(19.86% and 31.33%) was lower than that of EGFR and Ki67 negative patients (70.11% and 90.91%),P<0.05. The EGFR and Ki67 positive patients who had received adjuvant chemotherapy had a higher metastasis rate (70.37% and 62.50%) than EGFR and Ki67 negative patients(11.11% and 0),P<0.05. CONCLUSIONS: The aberrant expressions of EGFR and Ki67 in non-small cell lung cancer were highly correlated with tumor occurrence and development,and the expression of both presented co-ordination role. So the immunohistochemical expression of EGFR and Ki67 seems to be a useful tumor marker for patients with non-small cell lung cancer and can be used to evaluate the tumor biological behavior, sensitivity to the treatment and prognosis of the disease.