2-甲氧基雌二醇对大鼠神经元及C6胶质瘤细胞增殖与凋亡的影响

目的：探讨2-甲氧基雌二醇(2-methoxyestradiol，2-ME)对大鼠神经元及C6胶质瘤细胞增殖及凋亡的影响。方法：将神经元及C6胶质瘤细胞分为对照组和实验组，实验组按2-ME浓度不同分为1、5、10、15μmol/L 4个亚组。2-ME作用于大鼠神经元及C6胶质瘤细胞不同时间后，采用MTT法检测细胞活性，流式细胞仪检测细胞凋亡和细胞周期，并在光镜及电镜下观察其形态学变化。结果：C6胶质瘤细胞经2-ME作用后细胞活性受到抑制，呈现时间变量依赖性，各亚组与对照组差异具有统计学意义(P<0.05)。2-ME可诱导C6胶质瘤细胞凋亡，各亚组与对照组差异具有统计学意义(P<0.05)；可使C6胶质瘤细胞G₁及S期细胞减少，G₂期细胞增多，一定范围内呈现剂量依赖性，各亚组与对照组差异具有统计学意义(P<0.05)。神经元经药物作后，各亚组与对照组细胞活性差异无统计学意义。结论：2-ME可抑制C6胶质瘤细胞生长，诱导C6胶质瘤细胞凋亡，而对正常神经元无影响。

Effect of 2-methoxyestradiol on proliferation and apoptosis of neurons and C6 glioma cells in rats

Objective: To investigate the effect of 2methoxyestradiol on growth inhibition and apoptosis of C6 glioma cells and neurons in vitro.Methods: C6 glioma cells and neurons were divided into the control group and four 2-methoxyestradiol treatment groups.C6 glioma cells and neurons were treated by different doses of 2-methoxyestradiol for different times and observed by microscopy and electromicroscopy.The viability was determined by MTT assay and the cell-cycle and apoptosis were determined by flow cytometry.Results: 2-methoxyestradiol caused a dose and time-dependent inhibition on the C6 glioma cells viability(P<0.05).The population of C6 glioma cells in the G1 and S phase wasdecreased while in the G2 phase it was increased by 2-methoxyestradiol in a dose and time-dependent manner(P<0.05);However,2-methoxyestradiol had no effect on neurons.Conclusion: 2-methoxyesradiol can inhibit proliferation and induce apoptosis of C6 glioma cells,but has no effect on neurons.