| C6/IL-24对鼠脑胶质瘤血管生成的影响 |
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| 引用本文： | 刘英姿,张学新,张磊.C6/IL-24对鼠脑胶质瘤血管生成的影响[J].中国微侵袭神经外科杂志,2010,15(2):77-79. |
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| 作者姓名： | 刘英姿 张学新 张磊 |
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| 作者单位： | 河北医科大学第四医院神经外科,石家庄,050011 |
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| 基金项目： | 河北省科技厅2006年指导性计划项目(编号:062761308) |
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| 摘 要： | 目的探讨IL-24基因对荷瘤大鼠脑胶质瘤组织血管生成的影响。方法将24只SD大鼠随机分为C6/IL-24细胞组和C6细胞组,每组12只,分别于颅内尾状核部位接种转染IL-24基因的C6细胞(C6/IL-24)和鼠胶质瘤C6细胞。接种后21d取大鼠脑肿瘤组织,采用RT-PCR法检测大鼠脑肿瘤组织中VEGFmRNA的表达,免疫组化法检测VEGF和CD34的表达,并根据免疫组化结果计算两组肿瘤组织中的微血管密度(MVD)。结果RT-PCR结果显示:C6/IL-24细胞组VEGFmRNA表达水平明显低于C6细胞组(P0.01)。免疫组化法结果显示:C6/IL-24细胞组大鼠VEGF表达水平及CD34阳性MVD计数明显低于C6细胞组(P0.01)。结论IL-24基因转染后可抑制大鼠脑肿瘤组织中VEGF基因的表达,降低MVD,抑制肿瘤组织中新生血管的生长。
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| 关 键 词： | 神经胶质瘤 白细胞介素24 新生血管化 病理性 大鼠 |
Influences of C6/IL-24 on angiogenesis of rat glioma |
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| Liu Yingzi,Zhang Xuexin,Zhang Lei.Influences of C6/IL-24 on angiogenesis of rat glioma[J].Chinese Journal of Minimally Invasive Neurosurgery,2010,15(2):77-79. |
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| Authors: | Liu Yingzi Zhang Xuexin Zhang Lei |
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| Institution: | Liu Yingzi,Zhang Xuexin,Zhang Lei Department of Neurosurgery,No.4 Hospital,Hebei Medical University,Shijiazhuang,Hebei 050011,China |
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| Abstract: | Objec tive To investigate the angiogenesis effects of IL -24 gene in rat glioma. Methods Twenty-four SD rats were randomly divided into two groups, 12 each: rat glioma C6 cells (C6 group) and C6 cells with IL-24 gene transfection (C6/IL-24 group) were injected into rat caudate nucleus respectively. Tumors were harvested 21 d after cell injection, the expression of VEGF mRNA was detected by RT-PCR and the expressions of VEGF and CD34 protein were detected by immunohistochemistry. The microvessel density (MVD... |
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| Keywords: | glioma interleukin-24 neovascularization pathologic rats |
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