苗族药红禾麻黄酮类成分与环境因子的灰色关联度分析

目的:建立苗族药红禾麻药材中黄酮类成分槲皮黄酮-3-芸香苷·芦丁含量测定方法,运用灰色关联度法分析影响红禾麻药材品质产地差异性的主要环境因子,探讨环境因子对红禾麻药材品质形成机制的影响。方法:采用HPLC法,以槲皮黄酮-3-芸香苷·芦丁为对照品,流动相乙腈-甲醇-水(15∶15∶70),检测波长360 nm,测定不同产地红禾麻药材中槲皮黄酮-3-芸香苷·芦丁的含量;采用土壤养分测定仪测定不同红禾麻产地土壤中的铵态氮、速效磷、有效钾、p H和水分;通过距产地较近的气象部门查询和以全球卫星定位系统GPS、海拔表等测定地理气候因子,运用灰色关联度法对槲皮黄酮-3-芸香苷·芦丁含量与气候及土壤因子的相关性进行分析。结果:不同产地红禾麻药材槲皮黄酮-3-芸香苷·芦丁在0.094 6~4.969 mg·g~(-1),地理气候因子对红禾麻药材中槲皮黄酮-3-芸香苷·芦丁含量的积累影响较大。结论:建立的红禾麻药材黄酮类成分含量测定方法简便快捷,准确可行,可作为红禾麻药材的质量控制方法。并且通过对红禾麻中槲皮黄酮-3-芸香苷·芦丁含量与环境因子的灰色关联度分析明确了主导环境因子,为该药材的优良种质资源筛选及GAP规范化种植提供前期研究基础,同时也为苗药红禾麻药材质量标准的提升提供了实验依据。

Grey Correlation Degree Analysis Between Flavonoids Ingredient in Guizhou Miao Medicine Laportea bulbifera and Environmental Factors

Objective: To establish the content determination method for flavonoids ingredients (quercetin-3-rutinoside)in Guizhou Miao medicine Laportea bulbifera, find out the dominant environmental factors which impact the original differences of quality in Guizhou Miao medicine L. bifera by gray correlation analysis, and discuss the influence of environmental factors on formation mechanism of quality in medicinal materials L. bifera. Method: HPLC method was employed to measure the difference of quercetin-3-rutinoside content of Guizhou Miao medicine L. bifera from different producing areas, with acetonitrile-methyl alcohol-water (15:15:70) as the mobile phase. The wavelength was set at 360 nm. The ammonium nitrogen, available phosphorus, available potassium, pH and water in the soil of different producing areas were determined by soil nutrients tester; geographical climatic factors were obtained by consulting the meteorological department near the place of origin and by GPS and altitude meter. The correlation between flavonoids ingredient (quercetin-3-rutinoside) content and climate and soil factors was analyzed by grey correlation analysis. Result: The content of quercetin-3-rutinoside of L. bifera from different producing areaswas 0.094 6-4.969 mg·g^-1 due to the great influence of geographical climate factors. Conclusion: The established determination method for flavonoids contents of medicinal material of L. bifera is easy, fast, accurate and feasible. It can be used as the quality control method for medicinal materials L. bifera. The dominant environmental factors were ensured by gray correlation analysis. It provides preliminary experimental basis for selection of excellent germplasm resources and GAP standardized planting. It also provides experimental basis to improve the quality standard of the medicinal materials of Guizhou Miao medicine L. bifera.