| 干扰素－α对兔离体的卵巢颗粒细胞孕酮生成的影响 |
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| 引用本文： | 宿滨,王启发,姜良,马青年,姚兰春.干扰素－α对兔离体的卵巢颗粒细胞孕酮生成的影响[J].基础医学与临床,1994(3). |
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| 作者姓名： | 宿滨 王启发 姜良 马青年 姚兰春 |
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| 作者单位： | 哈尔滨医科大学实验动物学部 |
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| 摘 要： | 本研究应用离体培养的兔卵巢颗粒细胞观察了ＩＦＮ－ａ对孕酮生成的影响。细胞预培养２４ｈ后，用ＩＦＮ－ａ（５０～８００Ｕ／ｍｌ）处理培养的颗粒细胞１２ｂ刺激孕酮的生成并呈剂量依赖关系。在ＩＦＮ－ａ加入培养细胞后３、１２和２４ｈ．刺激作用非常明显，但刺激作用的幅度在３和１２ｈ较大，分别为对照组的２４和２０倍。此外，ＩＦＮ－ａ刺激离体兔颗粒细胞孕酮生成的作用被同时加钙螫合剂ＥＧＴＡ（１ｍｍｏｌ／Ｌ）或钙通道阻断剂异搏定（ｖｅｒ）２０和２００μｍｏｌ／Ｌ所抑制。本研究结果表明，ＩＦＮ－ａ刺激离体兔卵巢颗粒细胞孕酮的生成，其作用依赖于细胞外的Ｃａ２＋浓度。
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| 关 键 词： | 干扰素－a，兔颗粒细胞，孕酮Ca2＋ |
Effect of Interferon-a on Progesterone Production in Rabbit Ovarian Granulosa Cells |
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| Xu Bin Wang Qifa Jiang Liang Ma Qingnian Yao Lanchun.Effect of Interferon-a on Progesterone Production in Rabbit Ovarian Granulosa Cells[J].Basic Medical Sciences and Clinics,1994(3). |
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| Authors: | Xu Bin Wang Qifa Jiang Liang Ma Qingnian Yao Lanchun |
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| Institution: | Xu Bin Wang Qifa* Jiang Liang Ma Qingnian* Yao Lanchun* |
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| Abstract: | he present study was designed to examine the possible role of IFN-a in theregulation Of rabbit ovarian steroidogenesis,using isolated granulosa cells .Resultsshowed that IFN-a stimulated progesterone production in rabbit granulosa cells ina pose-and time-dependent manner and that the stimulatory effect of IFN-a wasblocked by condition of EGTA and verapamil.The results suggest that IFN-a playsa role in the regulation of rabbit ovarian steroidogenesis,via calcium dependent m-echanism. |
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| Keywords: | a interferon rabbit granulosa cells progesterone Ca2+ * Department of Phaiology Harbin Medical University Harbin |
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