喉鳞状细胞癌中脆性组氨酸三联体基因启动子甲基化及其蛋白的表达

目的：探讨喉鳞状细胞癌(LSCC)组织中脆性组氨酸三联体(FHIT)基因启动子甲基化状态及与FHIT蛋白表达的关系。方法：采用PCR-based酶切甲基化及免疫组织化学SP技术，检测41例LSCC及其相对应的喉正常粘膜(NSE)中FHIT基因启动子甲基化状态及其蛋白表达。结果：在NSE组中，无一例出现FHIT基因启动子甲基化，而在LSCC组中，有24．4％(10／41)出现甲基化，且甲基化状态与病理分级及淋巴结转移有关(P＜O．01)；NSE组及LSCC组FHIT蛋白阳性率分别为100．0％(41／41)和46．3％(19／41)，且与肿瘤TNM分期及淋巴结转移有关(P＜O．05)；FHIT基因启动子甲基化与蛋白阳性表达有明显的相关性(P＜O．01)。结论：FHIT基因启动子甲基化是其基因失活的重要机制之一，在LSCC的发生、发展中起重要作用。

Methylation of promoter and expression of FHIT gene in laryngeal squamous cell carcinoma

Objective:To study the relationship between status of methylation of FHIT gene promoter and expression of its protein in laryngeal squamous cell carcinoma (LSCC).Method:PCR-based restriction enzyme assay and immunohistochemical SP technique were used to detect the status of methylation of FHIT gene promoter and expression of its protein in 41 cases of LSCC and their matched normal squamous epithelium (NSE).Result:In NSE, there was no promoter methylation of FHIT gene, while in LSCC the rate of it was 24.4%(10/41), and it was related to the tumor pathological grade and lymph node metastasis(P<0.01). The positive rate of FHIT expression in NSE and LSCC was 100.0% (41/41) and 46.3% (19/41) respectively, and the expression of FHIT was related to the tumor TNM stage and lymph node metastasis (P<0.05).There was significant correlation between methylation of FHIT gene promoter and expression of its protein(P<0.01).Conclusion:Methylation of promoter might be one of important factors of inactivation of FHIT gene, and might play important role in carcinogenesis and progression of LSCC.