Kiss-1与结肠癌SW480细胞增殖的关系

目的以结肠癌细胞SW480为模型,初步探讨Kiss-1基因过表达与结肠癌细胞增殖的关系。方法采用脂质体转染的方法将Kiss-1/pEGFP-N1重组质粒转染至SW480细胞,上调结肠癌细胞中Kiss-1的表达水平,经G418筛选出稳定表达株；根据细胞分为3组：阴性对照组（转染pEGFP-N1空载体）、实验组（转染pEGFPN1-Kiss1）及空白对照组（未转染）。Western blot检测转染后细胞中Kiss-1表达量的变化；采用CCK8试验分析Kiss-1对SW480细胞增殖的抑制效果。结果成功将pEGFP-N1-Kiss-1重组质粒转染至SW480结肠癌细胞,western blot检测出阴性对照组和空白对照组中显示Kiss-1低表达,实验组Kiss-1表达量增加,CCK8试验表明实验组细胞增殖率明显低于对照组（P〈0.05）。结论 Kiss-1可抑制结肠癌细胞的增殖率。

Effect of Kiss-1 on the Proliferation of Colon Cancer Cell SW480

ObjectiveTo use colon cancer cell SW480 as a model,to explore effects of overexpression of Kiss-1 on proliferation of colon cancer cells.MethodsUsing liposome transfection,Kiss-1/pEGFP-N1 recombinant plasmid was transfected into SW480 cells,colon cancer Kiss-1 cells were upregulated,stably expressed cells were selected by G418;the cells were divided into 3 groups:negative control group (pEGFP-N1 transfection with empty vector),experimental group (with pEGFP-N1-Kiss1) and control group (without transfection).Western blot detection was used to detect the expression of Kiss-1 after transfection and CCK8 test was applied to analyze the inhibitory effect of Kiss-1 on the proliferation of SW480 cells.ResultspEGFP-N1-Kiss-1 recombinant plasmid was successfully transfected into the colorectal cancer cell line SW480,Western blot detected low expression of Kiss-1 in the negative control group and blank control group,while Kiss-1 in experimental group was increased;CCK8 test showed significantly lower rate of proliferation of cells in the experimental group than the control group (P<0.05).ConclusionKiss-1 can inhibit the proliferation of colon cancer cells rate.