Rag2敲除小鼠脏器重量、血液生理生化指标及免疫细胞的研究

目的检测SPF级Rag2敲除小鼠的脏器重量,血生理生化等生物学特性指标和免疫细胞。方法分别选5、10周龄的Rag2敲除(knockout,KO)小鼠测定主要脏器重量,并测定主要血生理、生化指标。选取6周龄Rag2 KO小鼠,应用流式细胞仪检测细胞免疫(T细胞-CD3+、T细胞亚群-CD4+、CD8+)、体液免疫(B细胞-CD19+、B220+)、NK细胞(NK1.1+)和粒细胞(CD11b+)进行检测。结果同性别NOD/SCID小鼠,10周小鼠脑、肺脏、脾脏、肝脏、心脏、双肾脏重、TBIL、WBC高于5周龄。同周龄的雌雄相比,雄性小鼠心脏、双肾脏、肝脏、脾脏重量、AST、ALP、A/G、GLU、PLT、PCT、WBC、LYM%高于雌性。Rag2 KO小鼠无T细胞(0.36±0.15)%、CD4+T细胞(0.21±0.06)%、CD8+T细胞(0.23±0.07)%、CD19+B细胞(0.28±0.04)%和B220+B细胞(2.03±0.42)%)。NK细胞比例为(24.13±3.62)%,粒细胞比例为(57.20±3.85)%。结论 Rag2 KO小鼠与C57BL/6J小鼠的主要生物学特性基本一致。周龄和性别因素对其脏器重量、血生理生化指标都有一定的影响。Rag2 KO小鼠T、B淋巴细胞缺失。

The study on organ weights, blood physiological and biochemical parameters and immune cells in Rag2 knockout mice

Objective To measure the organ weights , blood physiological and biochemical parameters , and immune function of Specific Pathogen Free （ SPF） Rag2 knockout （ KO） mice.Methods Rag2 knockout mice were selected at five and ten weeks , and the organ weights , blood physiological and biochemical parameters were observed .The percentages of CD3＋, CD4＋, CD8＋, CD19＋, B220＋, NK1.1＋, and CD11b＋were checked by FCM in Rag2 KO mice at six week of age in terms of its T , B lymphocyte function and NK cell activity .Results Among the same sex group of NOD/SCID mice, the weights of brain, lung, spleen, liver, heart, kidney, and the levels of TBIL, WBC in 10 weeks of age are higher than 5 weeks.At the same age, the weights of heart, kidney, liver, spleen, and the levels of AST, ALP,＆amp;nbsp;A/G, GLU, PLT, PCT, WBC, and LYM%in male mice are higher than females.The Rag2 KO mice lacks T cells（0.36 ±0.15）%、CD4＋T cells（0.21 ±0.06）%、CD8＋T cells（0.23 ±0.07）%、CD19＋B cells（0.28 ±0.04）%、B220＋B cells（2.03 ±0.42）%）.The percentage of NK cells is（24.13 ±3.62）%, and the percentage of granulocytes is （57.20 ±3.85）%.Conclusion The study suggests that the organ weights , blood physiological and biochemical parameters are affected by age and gender in Rag2 KO mice, which main biological characteristics are similar with C 57BL/6J mice.The Rag2 KO mice show the deficiency of T , B cells function .