BALB/c突变卷毛小鼠皮肤组织结构的分析

目的探讨BALB/c突变卷毛小鼠的皮肤组织结构与正常BALB/c小鼠之间是否存在突变差异。方法选择10 d、21 d、42 d和63 d四个不同日龄组的BALB/c突变卷毛小鼠和正常BALB/c小鼠每组各10只,观察两组小鼠被毛外观差异情况,取两小块皮肤组织,直接镜检毛囊结构和皮肤组织病理学检测。结果 BALB/c突变卷毛小鼠生长至10 d时被毛弯曲,能够与同日龄的正常小鼠加以鉴别。镜检观察21 d、42 d和63 d的BALB/c突变卷毛小鼠毛囊中毛发根数明显少于正常小鼠,被毛稀疏且毛发卷曲明显。皮肤组织病理学检测结果显示四个不同日龄组的BALB/c突变卷毛小鼠的毛囊数均显著少于正常小鼠。63 d的BALB/c突变卷毛小鼠处于生长期的毛囊中可见毛发弯曲的现象。结论 BALB/c突变卷毛小鼠的毛囊和皮肤组织结构与正常小鼠存在明显差异,说明其皮肤组织结构也发生了改变,这为今后研究突变卷毛小鼠的突变基因和模型应用提供了理论参考。

Analysis of skin tissue structure in BALB/c mutant culy mouse

Objective To investigate if there are differences in the skin tissue structure between BALB/c mutant mice with curly hair and normal BALB/c mice. Methods 10-d,21-d,42-d and 63-d old groups of BALB/c mutant curly mice and normal BALB/c mice were used in this study, 10 mice in each group. The gross appearance of the mice were observed.Hair follicle and skin structure were examined and compared by histopathology. Results Clothing hair of the BALB/c mutant curly mice became bent at 10 day after birth,and could be distinguished from normal mice of the same age.Histological observation showed that the number of hair follicles and hair roots of BALB/c mutant curly mice was significantly less than that of normal mice, and the sparse coat and hair curling characteristics were obviously kept to 21 d, 42 d and 63 d of age.Histopathological examination revealed that the number of hair follicles in the mice in the four age groups of BALB/c mutant curly mice was also significantly less than that of normal mice.At 63 days of age, bending curly hair growing in hair follicles were visible in the BALB/c mutant curly mice. Conclusion Hair follicles and the skin tissue structure in the BALB/c mutant curly mice and normal mice showed distinct differences.These results demonstrate that the skin structure of mutant mice is also changed, which can provide a theoretical reference for future research on the mouse curly hair mutant gene and model application.